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pEPOR1CB0021

Plasmid

#117557

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0020

Plasmid

#117556

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0019

Plasmid

#117555

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0018

Plasmid

#117554

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0012

Plasmid

#117533

Purpose

Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

eSaCas9 (no stop codon)

Use

CRISPR

Mutation

R499A,Q500K,R654A,G655R

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Native promoter dCas9

Plasmid

#113656

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Publication

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

Native promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

S. pyogenes Cas9 native promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Rab20 cds in pGem-T Easy

Plasmid

#80540

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab20 (rab20 Zebrafish)

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG317

Plasmid

#105407

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

4xmyc tag fused to Twin-Strep tag

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTEI037

Plasmid

#105413

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Hormone-binding domain (glucocorticoid receptor) fused to mEGFP

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG143

Plasmid

#105398

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

mYFP (GFP variant) fused to SV40 nuclear localization signal

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-sod2 guide

Plasmid

#107604

Purpose

An entry vector with U6a and U6c promoter driving sod2 guide RNAs expression

Depositor

Qing Deng

Publication

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod2 guide RNA sequences

Use

CRISPR

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-sod1 guide

Plasmid

#107603

Purpose

An entry vector with U6a and U6c promoter driving sod1 guide RNAs expression

Depositor

Qing Deng

Publication

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod1 guide RNA sequences

Use

CRISPR

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-uqcrc1 guide

Plasmid

#107602

Purpose

An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression

Depositor

Qing Deng

Publication

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

uqcrc1 guide RNA sequences

Use

CRISPR

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSC1

Plasmid

#91179

Purpose

T-DNA vector expressing TALENs targeting Medtr4g020620 and Medtr2g013650A

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALENs targeting Medtr4g020620 and Medtr2g013650A

Use

TALEN

Expression

Plant

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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PatoK-OG241

Plasmid

#72840

Purpose

NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.

Depositor

Chris Barnes

Publication

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoK

Expression

Bacterial

Promoter

PatoK

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PatoM-OG241

Plasmid

#72842

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

Depositor

Chris Barnes

Publication

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoM

Expression

Bacterial

Promoter

PatoM

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PatoK-LOG241

Plasmid

#72848

Purpose

NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.

Depositor

Chris Barnes

Publication

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoK

Expression

Bacterial

Promoter

PatoK

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Rab44 cds in pGem-T Easy

Plasmid

#86229

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

Rab44 (rab44 Zebrafish)

Use

In situ probe/other

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Rab22a cds in pGem-T Easy

Plasmid

#86223

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

Rab22a (rab22a Zebrafish)

Use

In situ probe/other

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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Rab8a cds in pGem-T Easy

Plasmid

#86221

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

Rab8a (rab8a Zebrafish)

Use

In situ probe/other

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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