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CL20_mEGFP_CUX1_NUTM1

Plasmid

#205800

Purpose

Express mEGFP-tagged fusion protein, CUX1_NUTM1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CUX1_NUTM1 (CUX1, NUTM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle-PR.Cre

Plasmid

#192933

Purpose

pShuttle.Cre encoding sgRNAs targeting Trp53 and Rb1 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pShuttle-PR.CC9

Plasmid

#192932

Purpose

pShuttle.CC9 encoding sgRNAs targeting Trp53 and Rb1 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-LETMD1_STOP

Plasmid

#161460

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

LETMD1 (LETMD1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-TAF15 No Stop

Plasmid

#84896

Purpose

DONOR vector for Gateway cloning of TAF15 No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

TAF15 (TAF15 Human)

Use

Gateway donor

Mutation

No Stop

Promoter

none

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR1A_mmFbxw7(res) (closed)

Plasmid

#160105

Purpose

Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway entry vector

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_SENP6_WT_V5

Plasmid

#83008

Purpose

Gateway Donor vector containing SENP6, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SENP6 (SENP6 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pZB246-T7-T+

Plasmid

#250903

Purpose

Expresses N-terminal His-tag thermally stabilized T7 RNAP under a lac-inducible promoter on Kanamycin Resistance Backbone

Depositor

Timothy Whitehead

Article

Baumer et al bioRxiv [Preprint]. 2025 Nov 12:2025.11.

Insert

Thermally Stable T7 RNA Polymerase

Tags

6xHis

Expression

Bacterial

Mutation

Arginine 31 to Aspartic Acid, Alanine 61 to Argin…

Promoter

pT5-lacUV

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-HA-5'-CUX1

Plasmid

#244324

Purpose

5' AAVLINK plasmid for CUX1 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

CUX1 (CUX1 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Neo-CMV>hCCNL2

Plasmid

#239219

Purpose

Expresses CCNL2 in mammalian cell lines

Depositor

Jason Sheltzer

Article

Julian et al bioRxiv 2025.08.03.668359; doi: https://

Insert

cyclin L2 (CCNL2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v5 (B.1.617.3)

Plasmid

#212540

Purpose

Encodes SARS-CoV-2 spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.4

Plasmid

#212519

Purpose

Encodes SARS-CoV-2 variant BA.4 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.4 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19I ,deltaL24P25P26 ,A27S ,delH69-V70 ,G142D ,V2…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) G496S (TCA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

Plasmid

#212477

Purpose

Encodes SARS-CoV-2 spike Q493R, G496S, Q498R, N501Y, Y505H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q493R, G496S, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Q493R, G496S, Q498R, N501Y, Y505H

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 AY.4.2

Plasmid

#212457

Purpose

Encodes SARS-CoV-2 spike T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A222V ,D614G ,P681R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A222V ,D614G ,P681R (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R ,T95I ,G142D ,Y145H ,deltaE156-F157+R158G ,A…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 AY.1

Plasmid

#212433

Purpose

Encodes SARS-CoV-2 spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 AY.2

Plasmid

#212434

Purpose

Encodes SARS-CoV-2 spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v2 (B.1.617.1 v2)

Plasmid

#212429

Purpose

Encodes SARS-CoV-2 spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 SAK006879 VOC-501Y.V2 (B.1.351v5)

Plasmid

#212391

Purpose

Encodes SARS-CoV-2 spike D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 SAK007134 VOC-501Y.V2 (B.1.351v6)

Plasmid

#212393

Purpose

Encodes SARS-CoV-2 spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N5…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 SA190 VOC-501Y.V2 (B.1.351v4)

Plasmid

#212389

Purpose

Encodes SARS-CoV-2 spike D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A701V for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge