We narrowed to 12,753 results for: CAR

1. pSL3936 (pDonor(AAVS1)_Pse)

   Plasmid

   #200903

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (AAVS1 NGS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_CamKIIa_mKate2

   Plasmid

   #96942

   Purpose

   To label mature excitatory iPSC-derived human neurons

   Depositor

   Oleksandr Shcheglovitov, Ricardo Dolmetsch

   Article

   Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

   Insert

   far-red fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E87D (please see depositor comments below)

   Promoter

   CaMKIIa

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. F40 tension sensor

   Plasmid

   #101252

   Purpose

   Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

   Depositor

   Carsten Grashoff

   Article

   Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

   Insert

   YPet(short)-F40-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-UBE2S-UBD

   Plasmid

   #66713

   Purpose

   Expresses the UBE2S-UBD fusion protein in E. coli

   Depositor

   David Komander

   Article

   Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

   Inserts

   e2-epf (UBE2S )
   isot (USP5 )
   

   Use

   Tags

   GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOS

   Plasmid

   #123189

   Purpose

   binary plant vector for transient fLUC (with intron) expression

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::fLUC-I::tNOS
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLGCV

   Plasmid

   #49862

   Purpose

   Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).

   Depositor

   Anne Glover, Jonathan Pettitt

   Article

   Lagido et al BMC Physiol. 2008 Apr 2;8:7. doi: 10.118

   Insert

   firefly luciferase luc+ fused to GFP (S65C)
   

   Use

   Tags

   GFP (S65C)

   Expression

   Worm

   Mutation

   Promoter

   sur-5

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

   Plasmid

   #139448

   Purpose

   Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag E2-crimson
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. QUAS-jGCaMP7s-T2A-tdTomato

   Plasmid

   #200010

   Purpose

   QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

   Depositor

   Carolyn McBride

   Article

   Zhao et al Nature. 2022 May;605(7911):706-712. doi:

   Insert

   3xP3-ECFP-jGCaMP7s-td-Tomato
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJR021

   Plasmid

   #198814

   Purpose

   Variant of yellow fluorescent protein for transient gene expression

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::destabilizedYFP::let-838 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   YFP with destabilized signal from mouse ornithine…

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-neo-hApg5-HA

    Plasmid

    #22948

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

    Insert

    Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1(+)mGAT1-0-GFP

    Plasmid

    #41662

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal hydrophobic isoleucine residue added a…

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. stabilized HP35 tension sensor module

    Plasmid

    #101251

    Purpose

    Expresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.

    Depositor

    Carsten Grashoff

    Article

    Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

    Insert

    YPet(short)-HP35st-mCherry
    

    Use

    Retroviral

    Tags

    His-tag and cysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME GFP-PCNA

    Plasmid

    #105977

    Purpose

    entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Stat5A-SH2

    Plasmid

    #46515

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat5A (STAT5A Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 582-693

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-zAsCpf1

    Plasmid

    #107185

    Purpose

    plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    AsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-GGG-HIS-CBM-Xmod-DocIII

    Plasmid

    #91698

    Purpose

    CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-HIS-CBM-Xmod-DocIII
    

    Use

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Stat5B-SH2

    Plasmid

    #46516

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat5B (STAT5B Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 593-692

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+ EB3-mKate2

    Plasmid

    #105940

    Purpose

    in vitro transcription

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. HS-PCNA-GFP

    Plasmid

    #105942

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRE-Tn5-136

    Plasmid

    #118522

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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