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pRDA052H_sgCh2

Plasmid

#229023

Purpose

Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA052H_sgFOCAD #2

Plasmid

#229024

Purpose

Expression of a EnAsCas12a guide targeting FOCAD

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

FOCAD gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_051d_sgCiChr2-2

Plasmid

#228940

Purpose

Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-HRAS(GV12)

Plasmid

#233181

Purpose

Retroviral expression of HRAS(G12V)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

HRAS(G12V) (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-Isl1-P2A-mRuby2

Plasmid

#233169

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mLhx3-P2A-mRuby2

Plasmid

#233175

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-mNgn2x3HA-P2A-mRuby2

Plasmid

#233157

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Mouse, Synthetic)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-Isl1-P2A-mRuby2

Plasmid

#233158

Purpose

Retroviral expression of Isl1 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Isl1-P2A-mRuby2 (Isl1 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-mLhx3-P2A-mRuby2

Plasmid

#233159

Purpose

Retroviral expression of mLhx3 with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mLhx3-P2A-mRuby2 (Lhx3 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mNgn2x3HA-P2A-mRuby2

Plasmid

#233163

Purpose

Retroviral expression of Ngn2x3HA with mRuby2 fluorescent reporter

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

mNgn2x3HA-P2A-mRuby2 (Neurog2 Mouse, Synthetic)

Use

Retroviral

Tags

x3HA

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Puro-DEST (JDW 925)

Plasmid

#229808

Purpose

A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGLAST-DEST (JDW 1009)

Plasmid

#229822

Purpose

A single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-zdgat2FLAG-SV40

Plasmid

#232141

Purpose

Expresses zebrafish dgat2 with a C-terminal 3xFLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat2 (dgat2 Zebrafish)

Use

Zebrafish expression

Tags

3xFLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pXMLC2-DEST (JDW 7)

Plasmid

#229833

Purpose

A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway destination vector

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pME-MCS (JDW 455)

Plasmid

#229832

Purpose

A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attL1/L2 flanked MCS

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-BFP (JDW 491)

Plasmid

#229834

Purpose

A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-StayGoldc4-Giantin (JDW 1355)

Plasmid

#229844

Purpose

A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

n2StayGoldc4

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-zdgat2-CDS-FLAG

Plasmid

#232142

Purpose

Gateway middle entry vector containing zebrafish dgat2 with a C-terminal 3xFLAG tag

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat2 (dgat2 Zebrafish)

Use

Gateway cloning middle entry

Tags

3xFLAG

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ HA BEX3

Plasmid

#231004

Purpose

Transient overexpression of human BEX3 in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

BEX3 (BEX3 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2 3xFLAG FEM1A

Plasmid

#231001

Purpose

Transient overexpression of mouse FEM1A in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

FEM1A (Fem1a Mouse)

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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