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pPICZ-pre-Ost1-pro-alphaf-Btl2

Plasmid

#117665

Purpose

Study secretion efficiency of Btl2

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-pro-alphaf-Btl2

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107321

Purpose

Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to SaCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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mei-S332 5.6Kb genomic DNA in Casper4

Plasmid

#112984

Purpose

P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo) (mei-S332 Fly)

Use

P insertion vector

Tags

GFP

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_25

Plasmid

#111638

Purpose

Funclib design PTE_25

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_25

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_26

Plasmid

#111639

Purpose

Funclib design PTE_26

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_26

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_27

Plasmid

#111640

Purpose

Funclib design PTE_27

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_27

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_28

Plasmid

#111641

Purpose

Funclib design PTE_28

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_28

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_36

Plasmid

#111642

Purpose

Funclib design PTE_36

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_36

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_27.14

Plasmid

#111643

Purpose

Funclib design PTE_27.14

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_27.14

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_4

Plasmid

#111646

Purpose

Funclib design ACS_4

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_4

Tags

6xHis

Expression

Bacterial

Mutation

see depositor comments below

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_9

Plasmid

#111647

Purpose

Funclib design ACS_9

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_9

Tags

6xHis

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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dPTE2

Plasmid

#111634

Purpose

PROSS stabilized PTE S5

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

dPTE2

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_5

Plasmid

#111635

Purpose

Funclib design PTE_5

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_5

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_13

Plasmid

#111636

Purpose

Funclib design PTE_13

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_13

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_24

Plasmid

#111637

Purpose

Funclib design PTE_24

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_24

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTG-mTDGa.0

Plasmid

#81048

Purpose

bacterial expression of murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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