We narrowed to 17,160 results for: REV
-
Plasmid#148728PurposeBacterial Expression of SceIF4E_25-213-K42AK168AK187ADepositorInsertSceIF4E_25-213-K42AK168AK187A (CDC33 Budding Yeast)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpH-SceIF4E_25-213-KKKKtoAAAA_AF
Plasmid#148729PurposeBacterial Expression of SceIF4E_25-213-KKKKtoAAAADepositorInsertSceIF4E_25-213-KKKKtoAAAA (CDC33 Budding Yeast)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K122I
Plasmid#195614PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activityDepositorInsertI-SceI
ExpressionBacterialMutationC2458G, A2826T, A2827TPromoterT7Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF1 (G180A)
Plasmid#175415PurposeEncodes His-MBP-TEV-ybbR-G180A eRF1DepositorInserteRF1 (SUP45 Budding Yeast)
TagsHis-MBP-TEV and ybbRExpressionBacterialMutationG180APromoterT7Available SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH2 #7
Plasmid#166102PurposeThis plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.DepositorArticleAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH1 #4
Plasmid#166101PurposeThis plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.DepositorArticleAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_RPL39
Plasmid#166078PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.DepositorInsertPromoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
UseCRISPRExpressionYeastPromoterTet-inducibleAvailable SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI763 p(Z)-mCherry + UBC9 sgRNA
Plasmid#164918PurposeCEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNADepositorInsertP(Z)-mcherry
UseCRISPRExpressionYeastPromoterP(Z), synthetic promoterAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pCSJ182
Plasmid#159723PurposeExpress Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCgDepositorInsertGID4 (VID24 Budding Yeast)
UseYeast two-hybrid assayTags3xflag and Gal4ADExpressionYeastMutationremove stop codonPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB58 (Prp16)
Plasmid#111282PurposePrp16 in pSE358(Trp)DepositorInsertPRP16
ExpressionYeastAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N4A 3xFLAG
Plasmid#111975PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.DepositorInsertHsh155 N4A 3x FLAG
Tags3xFLAGExpressionBacterial and YeastMutationT178A R181A R182A R186AAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415 Ret2 I155A
Plasmid#73888PurposeI155A mutationDepositorInsertRet2 I155A
ExpressionYeastMutationIle 155 AlaPromoterMet25Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415 Ret2 I149A
Plasmid#73887PurposeI149A mutationDepositorInsertRet2 I149A
ExpressionYeastMutationIle 149 AlaPromoterMet25Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 TAG
Plasmid#87603PurposeEncodes Prp5 SAT motif to TAG for expression in yeastDepositorAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 K335R
Plasmid#87299PurposeEncodes Y2H Gal4 activation domain for Hsh155 K335RDepositorInsertHSH155 (HSH155 Budding Yeast)
TagsGAL4 AD and HAExpressionBacterial and YeastMutationK335RAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-CLF1
Plasmid#87548PurposeEncodes Y2H Gal4 DNA binding domain for Clf1DepositorAvailable SinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 D450G
Plasmid#87303PurposeEncodes Y2H Gal4 activation domain for Hsh155 D450GDepositorAvailable SinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only