We narrowed to 19,744 results for: Cre

1. GPR182-DuET

   Plasmid

   #213272

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR182 (GPR182 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas-pML-1B_6xHis-Cas12m

   Plasmid

   #192282

   Purpose

   MmCas12m fused to His-tag for protein purification

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12m
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-BRDT

   Plasmid

   #65381

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRDT (BRDT Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GRM2-DuET

   Plasmid

   #213301

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GRM2 (GRM2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac3-EcLeu TAG-EGFP**

   Plasmid

   #228785

   Purpose

   EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcLeuRS
   EcLtR
   EGFP**
   

   Use

   Baculoviral

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcBac3-EcTrp-TGA-EGFP**

   Plasmid

   #228786

   Purpose

   EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTrpRS
   EcWtR
   EGFP**
   

   Use

   Baculoviral

   Expression

   Mammalian

   Mutation

   TGA suppressor

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT/TO/FLAG/HA-HDLBP

   Plasmid

   #154450

   Purpose

   Expression of FLAG/HA-tagged HDLBP

   Depositor

   Markus Landthaler

   Article

   Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

   Insert

   HDLBP (HDLBP Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRC54-DHX37-GFP

   Plasmid

   #192149

   Purpose

   express DHX37-GFP

   Depositor

   Sidi Chen

   Article

   Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

   Insert

   DHX37-GFP (Dhx37 Mouse)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-SKP1-T131A

   Plasmid

   #213482

   Purpose

   Expresses human SKP1 T131A mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-T131A (SKP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139089

    Purpose

    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CCR5-DuET

    Plasmid

    #213201

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXD023 - pLKO-EF1a-Puro-P2A-FLuc-WPRE

    Plasmid

    #192203

    Purpose

    Lenti-PL(Puro-Luciferase)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-PL(Puro-Luciferase)
    

    Use

    Lentiviral; Mammalian expression

    Mutation

    NA

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPRv2_sgCtrl#1

    Plasmid

    #174148

    Purpose

    Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-CECR2

    Plasmid

    #65385

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    CECR2 (CECR2 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCIG-FGFRdn

    Plasmid

    #80431

    Purpose

    Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Fibroblast growth factor receptor 1 (FGFR1 Chicken)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains aa 1–425

    Promoter

    beta-actin promoter

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

16. GPR12-DuET

    Plasmid

    #213260

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR12 (GPR12 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-enCas12a-2xNLS

    Plasmid

    #209021

    Purpose

    Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    enCas12a-2xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R/K548R

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23119

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSET-iGluSnFR4s

    Plasmid

    #234443

    Purpose

    Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s
    

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF1a_HNF1A_P2A_Hygro_Barcode

    Plasmid

    #120448

    Purpose

    Barcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HNF1A (HNF1A Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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