We narrowed to 9,520 results for: try

1. pCS2+ GFP-2xESP3I-IRES-mCherry

   Plasmid

   #230996

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Type

   Empty backbone

   Tags

   AcGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFP.E227

   Plasmid

   #138249

   Purpose

   Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   mCherry
   Terminator
   mTagBFP
   Terminator
   gfp
   Terminator
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P17_up1691, P20_992, and iP16_3622

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR59.4

   Plasmid

   #63175

   Purpose

   Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   OmpR (ompR )
   sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2R3z-BASI-ccdB-BSAI

   Plasmid

   #118389

   Purpose

   Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   ccdB
   

   Use

   Unspecified

   Mutation

   See Depositor Comments Section

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p221z-CAS9p-TagRFP-t35s

   Plasmid

   #118386

   Purpose

   Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   CAS9p-TagRFP
   

   Use

   CRISPR

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-Gal80ts

   Plasmid

   #51619

   Purpose

   vertebrate expression of full-length temperature-sensitive Gal80 with stop

   Depositor

   Joshua Bonkowsky

   Article

   Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

   Insert

   Gal80ts (GAL80 Budding Yeast)
   

   Use

   Vertebrate expression

   Mutation

   temperature sensitive (G203V, G323R)*

   Available Since

   March 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENT-L1-Kozak-lacZ-stop-L2

   Plasmid

   #186359

   Purpose

   Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   lacZ (lacZ Synthetic, E. coli)
   

   Use

   Expression of lacz reporter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SaCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122090

   Purpose

   U6 driven SaCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-M2-TLR2

   Plasmid

   #12291

   Depositor

   Bruce Beutler

   Article

   Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   TLR2 (Tlr2 Mouse)
   

   Tags

   FLAG (FLAG-M2)

   Expression

   Mammalian

   Mutation

   M82I and H202Y; signal peptide (aa1-23) replaced …

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pLG327

    Plasmid

    #128797

    Purpose

    Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Expression

    Bacterial

    Promoter

    Pxyl(s)

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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