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pCAG-MBP-Foldon-ATG9 (692-839)

Plasmid

#188087

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (692-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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H14-MBP-SUMO-Cys-Linker-BAF_L58R

Plasmid

#101780

Purpose

Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Tags

His14-MBP-SUMO

Expression

Bacterial

Mutation

L58R mutation

Promoter

T5

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEF086 MBP-CREB 127-135-His

Plasmid

#154075

Purpose

Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET28 MBP-TEV-Rat BCKDK-His

Plasmid

#232119

Purpose

For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.

Depositor

Ruth Sommese

Article

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

BCKDK (Bckdk Rat)

Use

Tags

6 x His, Maltose-Binding Protein (MBP), and Tev S…

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pOCC120-MBP-DDX3X(D354V)-monoGFP-His

Plasmid

#219986

Purpose

DDX3X(D354V) protein purification

Depositor

Simon Alberti

Insert

DDX3X(D354V) (DDX3X Human)

Use

Tags

MBP and monoGFP and 6xHis

Expression

Insect

Mutation

D354V

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pOCC120-MBP-DDX3X(G325E)-monoGFP-His

Plasmid

#219987

Purpose

DDX3X(G325E) protein purification

Depositor

Simon Alberti

Insert

DDX3X(G325E) (DDX3X Human)

Use

Tags

MBP and monoGFP and 6xHis

Expression

Insect

Mutation

G325E

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLIB-2STREP-HsHelz_1051-1942-GSSG-MBP_AG

Plasmid

#148795

Purpose

Mammalian Expression of HsHelz_1051-1942-GSSG

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1051-1942-GSSG (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG

Plasmid

#148829

Purpose

Mammalian Expression of HsNot9-V181E-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-V181E-shRNA2res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-T411EL415E_AG

Plasmid

#148778

Purpose

Mammalian Expression of HsNot4_275-575-T411EL415E

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-T411EL415E (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_275-575-F405D_AG

Plasmid

#148773

Purpose

Mammalian Expression of HsNot4_275-575-F405D

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_275-575-F405D (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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