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pHAGE-PTEN-C136F

Plasmid

#116617

Purpose

Lentiviral expression of PTEN C136F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

C136F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-C136R

Plasmid

#116618

Purpose

Lentiviral expression of PTEN C136R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

C136R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-D331Tfs*13

Plasmid

#116620

Purpose

Lentiviral expression of PTEN D331Tfs*13

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

D331Tfs*13

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-G127R

Plasmid

#116621

Purpose

Lentiviral expression of PTEN G127R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

G127R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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BRSK2 gRNA (BRDN0001146828)

Plasmid

#78025

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRSK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRSK2 (BRSK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRSK2 gRNA (BRDN0001147787)

Plasmid

#78027

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRSK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRSK2 (BRSK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.G129E

Plasmid

#81689

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

G129E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-hMLH1dn

Plasmid

#174827

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-hMLH1dn

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CDKN1B-Puro

Plasmid

#172611

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK19-W105M-IRES-ZsGreen

Plasmid

#68859

Purpose

Expresses Human CDK19 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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