We narrowed to 4 results for: en1

1. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   ...genes associated with dementia, such as APP and PSEN1, in most cases the intended HDR events were corrupted...probably due to concomitant NHEJ repair. At the PSEN1 locus, over 90% of the HDR edited alleles had unwanted...

2. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

   Type

   Blog Post

   ...length polymorphism analysis, allele-specific PCR, T7EN1 cleavage assay, sequencing, RT-qPCR, or immunoblotting...

3. TALENs for Endogenous Zebrafish Genes

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   Collection

   ...gria3a TALEN1258 & TALEN1260 TCGTCCAATAGCTTCTcagtcacgcacgcctgtGAGTTTCTGCTCTTTA gria3a TALEN1258 & TALEN1259...TCGTCCAATAGCTTCTcagtcacgcacgcctgTGAGTTTCTGCTCTTTA gria3a TALEN1295 & TALEN1260 TCGTCCAATAGCTTCTCagtcacgcacgcctgtGAGTTTCTGCTCTTTA...TGACAGCGTTTACTCCACtagaagaagctgtgcgGGAGGATTTCTTTTTATA hey2 TALEN1297 & TALEN1257 TCTTCCGTTTCCACATCCaccacatcccaacagagcAGCGGGAGCAGCAGTAAA...

4. Fluorescent Protein Guide: Empty Backbones

   Type

   Collection

   ...bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression SGFP2 495 512 30 Monomer...