We narrowed to 5,877 results for: nls

1. Intersectin I SH3 C domain (human)

   Plasmid

   #47415

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain C (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain C

   Promoter

   tac

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

2. CRY-tTA (B702)

   Plasmid

   #92032

   Purpose

   Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   tTA2 (TetR fused to VP64)

   Expression

   Mammalian

   Mutation

   NLS on CRY2 is mutated

   Promoter

   CMV

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAN1735

   Plasmid

   #127204

   Purpose

   Dual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLS

   Depositor

   Hana El-Samad

   Article

   Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   pGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Intersectin I SH3 D domain (human)

   Plasmid

   #47416

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain D (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain D

   Promoter

   tac

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3 mouse Bmf

   Plasmid

   #17244

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bmf (Bmf Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pCR1161

   Plasmid

   #111097

   Purpose

   Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCA gRNA (FANCA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR1110

   Plasmid

   #111096

   Purpose

   Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   BLM gRNA (BLM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223 PAK5 T538N

   Plasmid

   #60537

   Purpose

   Donor vector for human Pak5 T538N mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   Pak5 (PAK5 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   T538N

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223 PAK5 S573N

   Plasmid

   #60538

   Purpose

   Donor vector for human Pak5 S573N mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   Pak5 (PAK5 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   S573N

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCR1322

    Plasmid

    #111109

    Purpose

    Expresses FANCL_+_58468435.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCL gRNA (FANCL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCR1271

    Plasmid

    #111106

    Purpose

    Expresses FANCI_-_89787239.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCI gRNA (FANCI Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p27.pA.NGFR.mCMV.hPGK.Rab35(S22N).wpre[sequenced]

    Plasmid

    #226766

    Purpose

    Rab35(S22N)

    Depositor

    Ferdinando Pucci

    Article

    Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

    Insert

    rab35-mutant S22N (RAB35 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p25.pA.NGFR.mCMV.hPGK.Rab35(WT).wpre[sequenced]

    Plasmid

    #226764

    Purpose

    rab35 (WT)

    Depositor

    Ferdinando Pucci

    Article

    Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

    Insert

    rab35 (RAB35 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p26.pA.NGFR.mCMV.hPGK.Rab35(Q67L).wpre[sequenced]

    Plasmid

    #226765

    Purpose

    Rab35(Q67L)

    Depositor

    Ferdinando Pucci

    Article

    Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

    Insert

    rab35-mutant Q67L (RAB35 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB9v2

    Plasmid

    #210029

    Purpose

    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB9

    Plasmid

    #210036

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    puro-T2A-eGFP
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpCas9-NRCH-CGBE1

    Plasmid

    #198557

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3E-IRES-mTagBFP2-pA (JDW 1361)

    Plasmid

    #224535

    Purpose

    A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    IRES-FLAG-NLS-mTagBFP2-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHA850 - osm-10p::FynY531F

    Plasmid

    #139207

    Purpose

    Express activated Fyn kinase in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Y531F

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHA852 - mec-4p::FynY531F

    Plasmid

    #139209

    Purpose

    Expresses activated Fyn kinase in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Y531F

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FUS_LC_withR

    Plasmid

    #139128

    Purpose

    express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    add arginines

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TOG_D1

    Plasmid

    #139112

    Purpose

    express first domain (1-250) of TOG

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    TOG/CKAP5 (CKAP5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    1-250

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. hnRNPA2_LC_nocharge_wR

    Plasmid

    #139119

    Purpose

    express hnRNPA2 LC with no charged residues with R added back

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    remove all K, most D

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. hnRNPA2_LC_RtoK

    Plasmid

    #139120

    Purpose

    express hnRNPA2 LC where all R are K

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all R to K

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. hnRNPA2_LC_NtoS

    Plasmid

    #139121

    Purpose

    express hnRNPA2 with all N changed to S

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all N to S

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hnRNPA2_LC_5E

    Plasmid

    #139122

    Purpose

    express hnRNPA2 with 5 serine changed to glutamate

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    5 serine to glutamate

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hnRNPA2_LC_12E

    Plasmid

    #139123

    Purpose

    express hnRNPA2 with all serine change to glutamate

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    12 S to E

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. hnRNPF_PLD_YtoS

    Plasmid

    #139124

    Purpose

    express hnRNPF PLD with all Y changed to S

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all Y to S

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hnRNPF_PLD_StoA

    Plasmid

    #139125

    Purpose

    express hnRNPF PLD with all S changed to A

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all S to A

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCR1178

    Plasmid

    #111104

    Purpose

    Expresses FANCF_-_22647294.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCF gRNA (FANCF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCR1224

    Plasmid

    #111105

    Purpose

    Expresses FANCF_-_22647359.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCF gRNA (FANCF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCR1118

    Plasmid

    #111110

    Purpose

    Expresses HELQ_+_84376961.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    HELQ gRNA (HELQ Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCR1165

    Plasmid

    #111111

    Purpose

    Expresses HELQ_-_84376979.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    HELQ gRNA (HELQ Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCR1082

    Plasmid

    #111102

    Purpose

    Expresses FANCE_-_35420187.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCE gRNA (FANCE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCR1135

    Plasmid

    #111103

    Purpose

    Expresses FANCE_-_35420147.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCE gRNA (FANCE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. BPK4387 - human expression plasmid for SpCas9 Cluster 5

    Plasmid

    #101191

    Purpose

    Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster5(K918A/V922A/R925A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K918A/V922A/R925A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MMW3645 - human expression plasmid for SpCas9 Cluster 3

    Plasmid

    #101187

    Purpose

    Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    T657A/G658A/W659A/R661A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MMW3770 - human expression plasmid for SpCas9 Cluster 4

    Plasmid

    #101189

    Purpose

    Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    F491A/M495A/T496A/N497A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR223 PAK5 P50L

    Plasmid

    #60533

    Purpose

    Donor vector for human Pak5 P50L mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    P50L

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pDONR223 PAK5 P76T

    Plasmid

    #60534

    Purpose

    Donor vector for human Pak5 P76T mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    P76T

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

41. pDONR223 PAK5 S312C

    Plasmid

    #60535

    Purpose

    Donor vector for human Pak5 S312C mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    S312C

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

42. pDONR223 PAK5 V463L

    Plasmid

    #60536

    Purpose

    Donor vector for human Pak5 V463L mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    V463L

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

43. pDONR223 PAK5 V593I

    Plasmid

    #60539

    Purpose

    Donor vector for human Pak5 V593I mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    V593I

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

44. pAN1777

    Plasmid

    #127206

    Purpose

    Dual inducible expression of SynTF-mScarlet-nesSwitchA and KeyA-BFP-NLS

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pGal1-NLS(activity4)-SynTF-mScarlet-1_nesSwitch_A_t9-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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45. BII-STAR-tdT

    Plasmid

    #133396

    Purpose

    Piggybac vector for ASCL2 reporter

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Stem Cell Specific ASCL2 response elements with L…

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. STEME-4

    Plasmid

    #138140

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    wtTadA-TadA7.10-APOBEC3A-nCas9-2X(T2A-UGI-NLS)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. STEME-3

    Plasmid

    #138139

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice protoplasts

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-2X(T2A-UGI-NLS)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pH-STEME-1-esgRNA

    Plasmid

    #138135

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pH-STEME-NG-esgRNA

    Plasmid

    #138136

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid

    #107270

    Purpose

    Expresses Cas9 upon induction with doxicycline.

    Depositor

    Pablo Menéndez

    Article

    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

    Inserts

    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    

    Use

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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