We narrowed to 4,118 results for: 272

Showing: 1161 - 1180 of 4118 results

Results per page:

Sort results by:

Met133ILeu, Met186ILeu ELL2

Plasmid

#127264

Purpose

For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Two Mets (133,186 )to ILeu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met331Leu ELL2

Plasmid

#127262

Purpose

For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met331 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met331 to ILeu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
COOHend ELL2

Plasmid

#127255

Purpose

Expresses the C terminal segment of human ELL2 in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (C-terminal end) (ELL2 Human)

Use

Other

Expression

Mammalian

Mutation

contains only the last 389 aa

Promoter

pEF1a

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met1ILeu ELL2

Plasmid

#127258

Purpose

For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met1 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met1 to Ileu by Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ELL2mRNA+polyA

Plasmid

#127256

Purpose

For in vitro translation of human ELL2 with 30 nt pA tail

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

shortens 3'UTR to 40 bp adds A30 tail

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NH2end ELL2

Plasmid

#127252

Purpose

Expresses the N terminal segment of human ELL2 in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (1-287) (ELL2 Human)

Use

Other

Tags

His6X

Expression

Mammalian

Mutation

contains only amino acids 1-289

Promoter

pEF1a

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA tagged Xba ELL2

Plasmid

#127268

Purpose

For in vitro translation of WT ELL2 with HA tag inserted near N-term

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

Promoter

T7

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only
pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN1646

Plasmid

#127205

Purpose

pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1

Use

Synthetic Biology

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-NOD2-EGFP

Plasmid

#131207

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD2 (NOD2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
DRD5-Tango

Plasmid

#66272

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

DRD5 (DRD5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
nAChR beta2 WT

Plasmid

#24272

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

nAChR beta2 WT (Chrnb2 Mouse)

Expression

Mammalian

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-hs-HMGA2

Plasmid

#52727

Purpose

retroviral expression of human HMGA2

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HMGA2 (HMGA2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-U6-sgCTG

Plasmid

#232723

Purpose

SpCas9 guide RNA targeting CAG repeats for C-to-T base editing

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

SpCas9 gRNA targeting CAG repeats

Expression

Mammalian

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBJG1 T7-8xHis-OGT K842M

Plasmid

#154272

Purpose

Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi

Insert

O-GlcNAc Transferase (OGT Human)

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

Lysine 842 changed to Methionine- bacterial codon…

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-tetO-Ets2

Plasmid

#70272

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ets2 (Ets2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge