We narrowed to 22,307 results for: CRISPR

1. pHDE-35S-Cas9-mCherry-UBQ

   Plasmid

   #78932

   Purpose

   For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs

   Depositor

   Yunde Zhao

   Article

   Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKCcas9dO

   Plasmid

   #62552

   Purpose

   High efficiency Streptomyces genome editing by CRISPR/Cas9 system

   Depositor

   Weihong Jiang

   Article

   Huang et al Acta Biochim Biophys Sin (Shanghai). 201

   Inserts

   Scocas9
   sgRNA ()
   act-orf4 homology arm
   

   Use

   CRISPR; Bacterial genome editing

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   j23119 and ptipA

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-SpyCas9

   Plasmid

   #113033

   Purpose

   SpyCas9 expression in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SpyCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLdCN

   Plasmid

   #84290

   Purpose

   Express gRNA and Cas9 in Leishmania with Neomycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLdCH

   Plasmid

   #84291

   Purpose

   Express gRNA and Cas9 in Leishmania with Hygromycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCi-hTRAC-sgRNA

   Plasmid

   #154093

   Purpose

   pQCi backbone with sgRNA targeting human TCR-alpha common chain

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA targeting human TRAC locus
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCT

   Plasmid

   #60620

   Purpose

   Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

   Plasmid

   #216421

   Purpose

   Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   3xmyc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPbU6_hdhfr/yfcu

   Plasmid

   #216422

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459V2.0-eSpCas9(1.1)+K810A+K855A

    Plasmid

    #108299

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL2680

    Plasmid

    #85581

    Purpose

    Replicating base vector for constructing cpf1/CRISPR editing plasmids

    Depositor

    Himadri Pakrasi

    Article

    Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTFAM.1.0-gDNA

    Plasmid

    #132443

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TFAM (TFAM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. px330_SMAD exon 9 gRNA

    Plasmid

    #68352

    Purpose

    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Insert

    SMAD exon 9 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGRHL2.1.0-gDNA

    Plasmid

    #175863

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    GRHL2 (GRHL2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_HHEX_1

    Plasmid

    #72354

    Purpose

    Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    HHEX (HHEX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pARID4B.1.0-gDNA

    Plasmid

    #132436

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ARID4B (ARID4B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSRNP3.1.0-gDNA

    Plasmid

    #132472

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    CSRNP3 (CSRNP3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHOXB6.1.0-gDNA

    Plasmid

    #132458

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGTF2I.1.0-gDNA

    Plasmid

    #132452

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    GTF2I (GTF2I Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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