We narrowed to 3,114 results for: GAI

1. JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)

   Plasmid

   #156417

   Purpose

   8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   murine Dll4 F2-6/F8 ETS site B/ site #2, 8X
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   E1b min pro/b-globin intron

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-Lrrc40-trunc-IRES-BFP

   Plasmid

   #117268

   Purpose

   Retroviral overexpression of Lrrc40 - truncated version with seemingly same DE genes in Th2 as MSCV-Lrrc40-IRES-BFP

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Lrrc40 (Lrrc40 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The insert is truncated - deletion of bp 334-407 …

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75246

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Helios (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75247

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Helios (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGMC00030 (aka pRC0707)

   Plasmid

   #209673

   Purpose

   Lentiviral vector expressing PP7-P65-HSF1

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Insert

   PP7-P65-HSF1 (HSF1, RELA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-ASAP3Kv-WPRE

   Plasmid

   #132330

   Purpose

   expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3KV
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3-EF1a-YapS127A

   Plasmid

   #46049

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S127A

   Promoter

   EF1a

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pLY100-RetroEFS-HER2-28BBz-WPRE

   Plasmid

   #192201

   Purpose

   Retro-HER2CAR

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-HER2CAR (ERBB2 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7NT*-Bri2 113-231 R221E

   Plasmid

   #138134

   Purpose

   Expresseds human Bri2 BRICHOS R221E mutant in E. coli

   Depositor

   Jan Johansson

   Article

   Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

   Insert

   human Bri2 BRICHOS R221E (ITM2B Human)
   

   Use

   Tags

   NT* tag derived from spider silk proteins

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3-EF1aH Yap S127A

    Plasmid

    #86497

    Purpose

    Mammalian expression of FLAG-tagged Yap containing S127A mutation

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes associated protein 1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX458-sgEIF4EBP1

    Plasmid

    #128097

    Purpose

    CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    CRISPR sgRNA against human 4EBP1 (EIF4EBP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFBOH-MHL.RBBP4

    Plasmid

    #125173

    Purpose

    Expresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Histone-binding protein RBBP4 (RBBP4 Human)
    

    Use

    Tags

    His-TEV site

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNTK human MBD3 PGK-Neo-pA flox

    Plasmid

    #52362

    Purpose

    Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    MBD3 (MBD3 Human)
    

    Use

    Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

    Plasmid

    #156402

    Purpose

    Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW-Flag-Mbd3 delta1-70

    Plasmid

    #52372

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-70

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-Flag-Mbd3 delta 25-83

    Plasmid

    #52373

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    amino acids 25-83 deleted

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUW-Flag-Mbd3 delta49-63

    Plasmid

    #52374

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 49-63

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFBOH-MHL.EED

    Plasmid

    #125174

    Purpose

    Expresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Polycomb protein EED (EED Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. px458-CCND1

    Plasmid

    #172630

    Purpose

    Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against CCND1 (CCND1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. shERK2b-mlpx-puro

    Plasmid

    #65230

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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