Purpose
              
              This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
             
          
          
          
            
          
        
          
  
  
    
      
        Inserts
      
      
        
          EGFP
        
          Pyrrolysyl tRNA(cua)
        
          Pyrrolysyl tRNA synthetase (methanosarcina mazei)
        
      
    
   
  
  
    
      
        Use
      
      
        
          Replicative expression plasmid for cyanobacteria …
        
      
     
  
  
    
  
  
  
    
      
        Mutation
      
      Tyrosine  in position 35 of the GFP was mutated f…
     
  
  
    
      
        Promoter
      
      LeuP (native S. elongatus promoter), PpsbII, and …
     
  
         
          
            
              
                Available Since
              
              
                Oct. 11, 2017
              
             
          
          
            
              Availability
            
            
              
                Academic Institutions and Nonprofits only