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pZNpF-deleted

Plasmid

#245372

Purpose

Negative control, luciferase cassette deleted

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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ISA585: PM-42

Plasmid

#241970

Purpose

42 kb phagemid.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

PM-42

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCIneo-RLuc_C

Plasmid

#146090

Purpose

Mammalian Expression of Rluc

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

Rluc

Expression

Mammalian

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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p64T-XBMP2

Plasmid

#117253

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

Insert

BMP2

Use

Xenopus expression

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSP64T-BMP4

Plasmid

#117254

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

Insert

BMP4

Use

Xenopus expression

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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Ternary BAC Reporter

Plasmid

#78220

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCyc-Cdc24

Plasmid

#66574

Purpose

Express an additional copy of Cdc24, integrated at TRP locus

Depositor

Orion Weiner

Article

Jost et al ACS Synth Biol. 2015 Jun 2.

Insert

Cdc24

Expression

Yeast

Promoter

pCyc1

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pUC120

Plasmid

#50350

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Timmermans et al J Biotechnol. 1990 Jun;14(3-4):333-44.

Type

Empty backbone

Use

Cloning vector

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
pEGFPc1 IP3R3 WT

Plasmid

#236453

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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3xFlag-eGFP-Flag-SETX

Plasmid

#218838

Purpose

Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

Depositor

Albert La Spada

Article

Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

Insert

Senataxin (SETX Human)

Tags

Flag and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pENN236_CRISPRcharm_Kv2

Plasmid

#220839

Purpose

CRISPRcharm Kv2 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv2

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-3xFlag

Plasmid

#245989

Purpose

Express 3xFlag C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pENN239_CRISPRcharm_Kv1

Plasmid

#220838

Purpose

CRISPRcharm Kv1 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv1

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a spIgE-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-Puro

Plasmid

#248034

Purpose

Constitutively expresses 3xFLAG tagged Neoleukin 2/15 with an IgE signal peptide, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

3xFLAG-tagged Neoleukin-2/15

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pX458-Dual-Guide-Donor-ShuttleOnly

Plasmid

#171098

Purpose

Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Mutation

XbaI site added upstream of U6 promotor. GtoC mu…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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BOLL_RRM_pGEX

Plasmid

#135130

Purpose

Recombinant expression of BOLL with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

BOLL (BOLL Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pENN226_CRISPRcharm

Plasmid

#220834

Purpose

CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gnaq

Plasmid

#246001

Purpose

Express HA N-terminal tagged mouse Galphaq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnaq (Gnaq Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_WT-PolyA

Plasmid

#112284

Purpose

Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

WT human YAP1-2gamma

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-dCas9-T2A-GFP

Plasmid

#193781

Purpose

Tricistronic expression of dCas9-EGFP-BSD

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

dead Cas9

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

In frame with existing Cas9 promoter

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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