We narrowed to 3,098 results for: actin

1. lentiCRISPR v2-sgNCKAP1-2

   Plasmid

   #210134

   Purpose

   knock out NCKAP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Nck-associated protein 1 (NCKAP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR v2-sgNCKAP1-1

   Plasmid

   #210133

   Purpose

   knock out NCKAP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Nck-associated protein 1 (NCKAP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

   Plasmid

   #103031

   Purpose

   Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. aLL-EGFP

   Plasmid

   #103880

   Purpose

   aL-EGFP with 4aa linker

   Depositor

   Timothy Springer

   Article

   Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

   Insert

   full-length human aL with EGFP in betapropeller (ITGAL Human)
   

   Use

   Tags

   tension sensor module

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. aL-L3

   Plasmid

   #103881

   Purpose

   full-length human aL with moxsynGFP in betapropeller

   Depositor

   Timothy Springer

   Article

   Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

   Insert

   full-length human aL with moxsynGFP in betapropeller (ITGAL Human)
   

   Use

   Tags

   tension sensor module

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR

   Plasmid

   #196518

   Purpose

   Expression of Ctdnep1_D67E-GFP

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Ctdnep1_D67EsiR (CTDNEP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   D67E mutation (phosphastase dead). Silent mutatio…

   Promoter

   CMV promoter; T7 promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pE-cadherin-GFP-N-WASP deltaVCA

   Plasmid

   #101283

   Purpose

   Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   delta VCA domain

   Promoter

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Moe-pDONR221

   Plasmid

   #124239

   Purpose

   Gateway entry clone of Drosophila Moesin cDNA encoding the D isoform.

   Depositor

   Peter Vilmos

   Article

   Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

   Insert

   Drosophila Moesin (Moe Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7-EF-EYFP-YAP1_Y357F-PolyA

   Plasmid

   #112287

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoform

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Tyrosine 357 to Phenyalanine corresponding to tyr…

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-6P-1-Eps8L2_1-496

    Plasmid

    #196516

    Purpose

    Expression of GST-Eps8L2_1-496

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2_1-496 (EPS8L2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Silent mutations for siRNA resistance

    Promoter

    lac promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Nmyc-Eps8L2_1-496-siR

    Plasmid

    #196515

    Purpose

    Expression of Myc-Eps8L2_1-496

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2_1-496 (EPS8L2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Silent mutations for siRNA resistance

    Promoter

    T7 promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

    Plasmid

    #193719

    Purpose

    Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
    

    Use

    Tags

    2xHA-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid

    #193721

    Purpose

    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)
    

    Use

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Nmyc-Eps8L2-FL-siR

    Plasmid

    #196514

    Purpose

    Expression of Myc-Eps8L2-FL

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2-FL-siR (EPS8L2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Silent mutations for siRNA resistance

    Promoter

    T7 promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLL3.7-EF-EYFP-YAP1_R89A-PolyA

    Plasmid

    #112291

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with Arginine 89 to Alanine

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL3.7-EF-EYFP-YAP1_L91A-PolyA

    Plasmid

    #112292

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with Leucine 91 to Alanine

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL3.7-EF-EYFP-YAP1_F95A-PolyA

    Plasmid

    #112293

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with Phenylalanine 95 to Alanine

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJC20cdc8.A18T

    Plasmid

    #99360

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJC20cdc8.E31K

    Plasmid

    #99361

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Glutamic acid 31 to Lysine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJC20cdc8.110

    Plasmid

    #99363

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine and Glutamic acid 31 to L…

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge