We narrowed to 5,134 results for: golden gate

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pBTK1051

Plasmid

#209541

Purpose

BTK Type 5 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1056

Plasmid

#209546

Purpose

BTK Type 4 antibiotic resistance cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Type 4 Kanamycin resistance cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNL2119 pARF7 GG0-GG1

Plasmid

#195910

Purpose

Level 0 pARF7 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF7 (NPH4 Mustard Weed)

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pNL2080 pGATA23 GG0-GG1

Plasmid

#195908

Purpose

Level 0 pGATA23 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pGATA23

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I39 PhiC31-Intein GG1-GG4

Plasmid

#195905

Purpose

Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31-intein

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I32 pLBD16 GG0-GG1

Plasmid

#195903

Purpose

Level 0 pLBD16 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pLBD16

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I9 PhiC31 GG1-GG4

Plasmid

#195892

Purpose

Level 0 PhiC31 integrase with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I40 Intein-PhiC31 GG1-GG4

Plasmid

#195906

Purpose

Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Intein-PhiC31

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pNM3746

Plasmid

#154109

Purpose

loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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BCJJ223

Plasmid

#117509

Purpose

AtMGE1 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pMGE1:AATG:BsaI

Expression

Plant

Promoter

AtMGE1

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL12021

Plasmid

#117511

Purpose

AtICU2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pICU2:AATG:BsaI

Expression

Plant

Promoter

AtICU2

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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LBJJ433

Plasmid

#117507

Purpose

AtEC1.2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pEC1.2:AATG:BsaI

Expression

Plant

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ133D

Plasmid

#69289

Purpose

Golden Gate entry vector; 1st gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLOXp-GUS

Plasmid

#154059

Purpose

Level 0.5 Golden Gate vector, loxP-GUS-nosT-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

beta-glucoronidase (E. coli)

Use

Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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LbCas12a-gRNA_BbsI_CAM

Plasmid

#183222

Purpose

LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2259 - Intron GG2 - 900 bp

Plasmid

#159881

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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EC71173

Plasmid

#154061

Purpose

Level 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-Cre

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

Cre recombinase

Expression

Bacterial

Mutation

Introduction of U5 intron sequences, to prevent p…

Promoter

HvHSP17

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSN007

Plasmid

#102440

Purpose

For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

Depositor

Alejandro Chavez, George Church

Article

Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

Insert

7SK paired gRNA insert

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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BCJJ344

Plasmid

#117503

Purpose

UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtUBI10

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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