We narrowed to 5,501 results for: golden gate
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Plasmid#228894PurposeGolden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
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SM425_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-KAN-DR
Plasmid#244225PurposeVector for golden gate cloning LbCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceNov. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceExpressionWormPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LBJJ432
Plasmid#117508PurposeAtEC_enh. promoter Golden Gate Level 0DepositorInsertBsaI:GGAG:pEC_enh:AATG:BsaI
ExpressionPlantPromoterEC1.2:EC1.1Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGD_MiniTurboID-YFP
Plasmid#222434PurposeGolden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GG-dest
Plasmid#69538PurposeDestination plasmid for gRNA concatenation Golden Gate cloningDepositorTypeEmpty backboneAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PBgg-Hygro
Plasmid#248968PurposeGolden Gate assembly-friendly PiggyBac backbone with Hygromycin resistanceDepositorTypeEmpty backboneExpressionMammalianAvailable sinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
GGDestX1-Amp
Plasmid#157652PurposeGolden Gate assembly and cell-free (bacterial) expression of fusion-proteinsDepositorTypeEmpty backboneUseCell-free expression (e. coli sigma-70)ExpressionBacterialPromoterOR2-OR1-PrAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132D
Plasmid#69288PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGB_YFP-MiniTurboID
Plasmid#222433PurposeGolden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
IBMc180
Plasmid#161942PurposeCarries Golden Gate substitution insert for acVHH-assisted bisection mappingDepositorInsertpSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
ExpressionBacterialPromoterPBADAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc050
Plasmid#161938PurposeCarries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
ExpressionBacterialPromoterPlux2Available sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc318
Plasmid#161941PurposeCarries Golden Gate substitution insert for ER-LBD domain-insertion mappingDepositorInsertpSB1A3-BbsI-G-ER-LBD-S-SapI
ExpressionBacterialAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131D
Plasmid#69287PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ2359 - intron GG2 - 250bp
Plasmid#159878PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG2 - 250bp
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
BCJJ339
Plasmid#117501PurposeRPS5a:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRExpressionPlantPromoterAtRPS5aAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC71022
Plasmid#159608PurposeLevel 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxPDepositorInsertloxP-mCherry-HDEL-t35S-loxP
UseCre/Lox and Synthetic BiologyMutationAll BsaI, Esp3I and BPiI restriction sites were r…Available sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
BCJJ180
Plasmid#117516PurposeCas9_4 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_4:GCTT:BsaI
UseCRISPRTags3xFLAG and NLSExpressionPlantAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP1950
Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBlue-TAL
Plasmid#49401PurposeThe pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.DepositorTypeEmpty backboneUseTALENExpressionInsectAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by