We narrowed to 3,788 results for: golden gate

1. U6m

   Plasmid

   #121019

   Purpose

   MoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

   Depositor

   Richard Murray

   Insert

   BCD20
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. U7m

   Plasmid

   #121020

   Purpose

   MoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

   Depositor

   Richard Murray

   Insert

   BCD16
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. U8m

   Plasmid

   #121021

   Purpose

   MoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).

   Depositor

   Richard Murray

   Insert

   BCD24
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. C84m

   Plasmid

   #120995

   Purpose

   MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Sigma 28
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C95m

   Plasmid

   #121001

   Purpose

   MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   T7 RNA polymerase (Andy please check!)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. C63m

   Plasmid

   #120979

   Purpose

   MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   lacI
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. C3m

   Plasmid

   #120956

   Purpose

   MoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)

   Depositor

   Richard Murray

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. P30m

   Plasmid

   #120942

   Purpose

   MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   pL-lacO1
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. C79m

   Plasmid

   #120991

   Purpose

   MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ccdA antitoxin
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. V37m

    Plasmid

    #121043

    Purpose

    MoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.

    Depositor

    Richard Murray

    Insert

    Kan destination vector with ColE1 origin and mScarlet selection
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. C83m

    Plasmid

    #120994

    Purpose

    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    pir protein required for R6K ori
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. C24m

    Plasmid

    #120964

    Purpose

    MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    lambda-cI
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. C27m

    Plasmid

    #120965

    Purpose

    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    tetR (no deg tags)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C98m

    Plasmid

    #121002

    Purpose

    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    Gp2 toxin
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C51m

    Plasmid

    #120975

    Purpose

    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file

    Depositor

    Richard Murray

    Insert

    sfYFP (has BbsI site)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C33m

    Plasmid

    #120968

    Purpose

    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    AiiA degradase (Bennett, no ssra tag)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. P57m

    Plasmid

    #120949

    Purpose

    MoClo golden gate assembly AB part for pCin* (Adam Meyer's improved pCin promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).

    Depositor

    Richard Murray

    Insert

    pCin Adam Meyer
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKS1604

    Plasmid

    #239528

    Purpose

    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    ScYea4p-mTurquoise2-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKS1606

    Plasmid

    #239529

    Purpose

    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    ScYea4p-mTurquoise2-2xCrk_SH3
    

    Expression

    Yeast

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDest_PANCEd_AraC_S170_LOV_sd2_WT

    Plasmid

    #242151

    Purpose

    Expression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170

    Depositor

    Dominik Niopek

    Insert

    AraC S170 LOV
    

    Expression

    Bacterial

    Available Since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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