We narrowed to 15,769 results for: LEA

1. pLY171

   Plasmid

   #184145

   Purpose

   sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   sgRNA-LEA2-ex3
   

   Use

   Synthetic Biology

   Promoter

   Plux2

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p663-UBC-miniTurbo-V5-HIPK4_IDG-K

   Plasmid

   #158185

   Purpose

   Gateway destination clone of HIPK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-HIPK4 (HIPK4 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   A670T, H813Y- please see depositor comment

   Promoter

   Ubiquitin

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FnRPR9-10

   Plasmid

   #182568

   Purpose

   Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 9-10
   

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1374A, P1376A, R1379A

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. FnRPR8-10

   Plasmid

   #182570

   Purpose

   Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 8-10
   

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1374A, P1376A, R1379A

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mouse KANK4-Y801H Ankyrin Repeats pET151

   Plasmid

   #164058

   Purpose

   Expresses mouse KANK4 Ank domain -Y801H mutant in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEL2114

   Plasmid

   #175961

   Purpose

   For expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Inserts

   LetA
   LetB
   

   Tags

   8xHis 2xQH and TEV cleavage site on LetA

   Expression

   Bacterial

   Mutation

   Δ634-746

   Promoter

   araBAD

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pElectra1-LaminA

   Plasmid

   #179482

   Purpose

   Nuclear envelope labeling with blue fluorescent protein Electra1

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   Electra1-LaminA
   

   Tags

   Electra1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4_HA-Halo-cmyc-GRM3 C127A

   Plasmid

   #140458

   Purpose

   Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Tags

   Halo, Signal/leader sequence from influenza hemag…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, Threonine 174 to alanine

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS2408-Tier1-PhCMV-TEVsite

   Plasmid

   #169503

   Purpose

   Tier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven tobacco etch virus protease cleavage site
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2409-Tier1-PhCMV-FURINsite

    Plasmid

    #169488

    Purpose

    Tier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Furin cleavage site
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-PAG-E2-Crimson-3XNLS-pA

    Plasmid

    #183801

    Purpose

    AAV vector to express nuclear localized E2-Crimson from PAG promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    E2-Crimson-NLS
    

    Use

    AAV

    Tags

    NLS

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CTAG_linker-N7

    Plasmid

    #136102

    Purpose

    Nuclear targeting peptide for C-term fusion with CDS12 without linker.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_AlaSer4xAla_N7
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. CTAG_NLS-SV40

    Plasmid

    #136108

    Purpose

    CTAG Nuclear localiation signal SV40 for fusion with CDS12

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_NLS-SV40
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pEPQD0CM0705

    Plasmid

    #177035

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    phiC31 integrase plus SV40 nuclear localization sequence (PKKKRKV)
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-sfGFP-pA-FRT-Amp-FRT

    Plasmid

    #176620

    Purpose

    for generating PCR templates with sfGFP and Amp for BAC recombination

    Depositor

    Nathan Lawson

    Article

    Shih et al Development. 2021 Nov 9. pii: 273393. do

    Insert

    super folder green fluorescent protein with FRT-flanked ampicillin cassette
    

    Use

    Unspecified

    Mutation

    H217R - please see dep. comments

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-pleCopyCatcher

    Plasmid

    #174061

    Purpose

    CopyCatcher insertion donor for the pale locus

    Depositor

    Ethan Bier

    Article

    Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

    Insert

    Pale (Pale Synthetic, Fly)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJCC_084 Cas12i1 D647A

    Plasmid

    #171671

    Purpose

    For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1 D647A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 647 to alanine

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET19b-rDAD2 cPGFP(N180)

    Plasmid

    #166001

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(N180)
    

    Expression

    Bacterial

    Mutation

    LSSmOrange- V49A (please see depositor comments)

    Promoter

    T7 promoter

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. NTTVTFLPTPSLILQ_epitope

    Plasmid

    #165020

    Purpose

    Expression of CMV UL44 (37-51) fused to CD74

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV UL44 (37-51)
    

    Use

    Lentiviral

    Tags

    CD74 at N-term; cathepsin S cleavage site (GRWHTV…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE

    Plasmid

    #159941

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2-optoNES
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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