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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 30,936 results for: LIS;

Showing: 11781 - 11800 of 30936 results

  1. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82702
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82702-plasmid-map-sequence-id-163793

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  2. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82701
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82701-plasmid-map-sequence-id-157303

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  6. pQC V5 mKO2 HuR.DelHNS IRES Puro

    Plasmid
    #110389
    Purpose
    γ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    V5 and mKO2
    Expression
    Mammalian
    Mutation
    HNS (HuR nuclear-cytoplasmic shuttling sequence) …
    Promoter
    CMV
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110389-plasmid-map-sequence-id-227209

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  7. pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500

    Plasmid
    #92230
    Purpose
    Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    NTerm 6xHis Tagged zmADH
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue V86 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available Since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92230-plasmid-map-sequence-id-181882

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  9. pLentiCMV_HyPer7RgDAAO

    Plasmid
    #217653
    Purpose
    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Use
    Lentiviral
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    CMV
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217653-plasmid-map-sequence-id-441459

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  10. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid
    #111573
    Purpose
    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells
    Depositor
    Thomas Muir
    Article
    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038
    Insert
    PARP1 (PARP1 Human)
    Tags
    C-terminal 6xHis and C-terminal Flag
    Expression
    Insect
    Mutation
    lysine to glutamine mutation at the following ami…
    Promoter
    polyhedrin promoter
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111573-plasmid-map-sequence-id-215588

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Showing: 11781 - 11800 of 30936 results