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We narrowed to 65,855 results for: nin

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Showing: 11881 - 11900 of 65855 results

  1. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid
    #140004
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140004-plasmid-map-sequence-id-275398

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  3. G protein alpha-s(alpha3beta5)

    Plasmid
    #55799
    Purpose
    This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.
    Depositor
    Catherine Berlot
    Publication
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T in alpha-s sequ…
    Promoter
    CMV
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    55799-plasmid-map-sequence-id-119340

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  4. pUASp YFP Rac1 DN

    Plasmid
    #52234
    Purpose
    Drosophila expression of YFP-Rac1 dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T17N; DN mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-489173

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  6. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid
    #114086
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    114086-plasmid-map-sequence-id-231034

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  7. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #106895
    Purpose
    Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    106895-plasmid-map-sequence-id-201653

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  8. pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR

    Plasmid
    #111502
    Purpose
    Expresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].
    Depositor
    Michael Lin
    Publication
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    ZF(VEGFA)-StaPL(AI)-YFP-VPR
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries V36M, T54A, and S122…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111502-plasmid-map-sequence-id-214269

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  15. pαH-S-GSAS-OMICRON

    Plasmid
    #180423
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    180423-plasmid-map-sequence-id-354264

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  16. pTRE2 EGFP

    Plasmid
    #129435
    Purpose
    express GFP
    Depositor
    Oskar Laur
    Insert
    GFP
    Expression
    Mammalian
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129435-plasmid-map-sequence-id-256213

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  18. pTXB1 EGFP

    Plasmid
    #129442
    Purpose
    expression of intein/ gfp
    Depositor
    Oskar Laur
    Insert
    intein/ gfp
    Expression
    Bacterial
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129442-plasmid-map-sequence-id-256468

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  20. R777-E211 Hs.RAC3

    Plasmid
    #70495
    Purpose
    Gateway ORF clone of human RAC3 [NM_005052.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RAC3 (RAC3 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70495-plasmid-map-sequence-id-125944

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Showing: 11881 - 11900 of 65855 results