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pMXs-hs-HRAS

Plasmid

#52730

Purpose

retroviral expression of human HRAS

Depositor

Shinya Yamanaka

Publication

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HRAS (HRAS Human)

Use

Retroviral

Expression

Mammalian

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-41

Plasmid

#172757

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUC19_CoV-2_ frD_ΔE3/F Δ67 DS Cav1 ΔCT

Plasmid

#253163

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; pre-fusion stabilized RSV F replaces orf3a and envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with RSV preF

Expression

Bacterial

Available since

Oct. 1, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_S Omicron BA.4/5

Plasmid

#253118

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), Wuhan backbone with Omicron BA.4/5 spike; chimeric

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron BA.4/5 spike

Expression

Bacterial

Available since

Sept. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pME-zfCre

Plasmid

#214939

Purpose

Gateway middle entry containing zebrafish codon-optimized Cre recombinase

Depositor

Nathan Lawson

Publication

Shin et al Dev Cell. 2023 Nov 20;58(22):2614-2626.e

Insert

zebrafish codon-optimized Cre

Use

Gateway Cloning

Available since

Sept. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pRUTH5-GST_SNRPD3-StrepII

Plasmid

#258260

Purpose

E. coli expression of human SNRPD3 C-terminal domain with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD3 (SNRPD3 Human)

Tags

6xHis-GST-TEV and Strep-tag II

Expression

Bacterial

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pRUTH5-GST_SNRPD1-StrepII

Plasmid

#258259

Purpose

E. coli expression of human SNRPD1 C-terminal domain with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD1 (SNRPD1 Human)

Tags

6xHis-GST-TEV and Strep-tag II

Expression

Bacterial

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pRUTH5-GST_SNRPB-StrepII

Plasmid

#258258

Purpose

E. coli expression of human SNRPB C-terminal domain with an N-terminal cleavable His-GST tag and a C-terminal Strep-tag II

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPB (SNRPB Human)

Tags

6xHis-GST-TEV and Strep-tag II

Expression

Bacterial

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2 frD_ΔrE/GFP

Plasmid

#253120

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope (residual AA). GFP is inserted in the locus of envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔE-GFP

Expression

Bacterial

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD ΔS/VSVG ΔE67 3*

Plasmid

#253173

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike, envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces spike

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with VSV-G

Expression

Bacterial

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/VSVG Δ67a

Plasmid

#253172

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with VSV-G

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/N1 PR8 Δ67a

Plasmid

#253169

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza N1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza N1 PR8

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD_Δ3E/H1 PR8 Δ67a

Plasmid

#253168

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; Influenza H1 (PR8) replaces orf3a and envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with Influenza H1 PR8

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2 ΔE7 3* Δ6/H1 PR8

Plasmid

#253147

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (PR8) replaces orf6

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 PR8

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2 ΔE7 3* Δ6/H1 WSN

Plasmid

#253146

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (WSN) replaces orf6

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza H1 WSN

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD ΔS/GFP

Plasmid

#253144

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike, GFP is inserted at the locus of Spike

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD ΔS/GFP

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_XBB_ΔE67 3*

Plasmid

#253122

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a, premature stop codon in orf3a at AA213. GFP is inserted in the locus of env

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔEΔ6Δ7 orf3*

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ΔE/GFP Δ68

Plasmid

#253121

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope (residual AA) , orf6, orf8. GFP is inserted in the locus of envelope

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔE-GFPΔ6Δ8

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_S Omicron BA.2

Plasmid

#253117

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), Wuhan backbone with Omicron BA.2 spike; chimeric

Depositor

Thomas Klimkait

Publication

Kipfer et al eLife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron BA.2 spike

Expression

Bacterial

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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