We narrowed to 18,078 results for: comp

1. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #135012

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG

   Plasmid

   #81023

   Purpose

   Cancer-associated ASXL1 truncation mutant in retroviral vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains ASXL1 amino acids 1-479

   Promoter

   5’LTR

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5/FRT/TO HIS HSPH2

   Plasmid

   #19535

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 4 (HSPA4 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   mutations A381T, T732A, and an insertion of "…

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-sgTSC2

   Plasmid

   #128098

   Purpose

   CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   sgRNA against human TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-ZNF251

   Plasmid

   #101451

   Purpose

   Donor Vector containing ZNF251 transcription factor, part of the Human TFome Collection

   Depositor

   George Church

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF251 (ZNF251 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-ubiquitin-EphB4-SV40-GFP (B4G)

   Plasmid

   #65444

   Purpose

   Expression of human EPHB4 and GFP in mammalian cells

   Depositor

   Eduard Batlle

   Article

   Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

   Insert

   EPHB4 (EPHB4 Human)
   

   Use

   Lentiviral

   Tags

   SV40-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   UBQ

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. shBCL2L1 # 2

   Plasmid

   #42552

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shBCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

8. shBCL2L1 # 1

   Plasmid

   #42551

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shBCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

   Plasmid

   #181956

   Purpose

   Genetically encoded FRET-based cAMP indicator fused to AKAP79.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
   

   Use

   Tags

   AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-Con/Foff 2.0-mCherry

    Plasmid

    #137133

    Purpose

    Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. psiCHECK-VIM270-gCM

    Plasmid

    #215868

    Purpose

    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

    Depositor

    Kumiko Ui-Tei

    Article

    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

    Insert

    target site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pATP416-­ptetO7-­pphlO6-­crtYBI

    Plasmid

    #165976

    Purpose

    Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pphlO6), Synthetic promoter (…

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSNAP-CAPZ

    Plasmid

    #69948

    Purpose

    Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

    Depositor

    Jeff Gelles

    Article

    Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

    Inserts

    CapZ beta 1 (CAPZB Chicken)
    CapZ alpha 1 (CAPZA1 Chicken)
    

    Use

    Tags

    His 6 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-ZNF706

    Plasmid

    #88037

    Purpose

    Donor Vector containing ZNF706 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF706 (ZNF706 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAR010 Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD

    Plasmid

    #195543

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG059 MBP-CREB-His

    Plasmid

    #154076

    Purpose

    Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CREB1 (CREB1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRepair-mScI-CTNNB1

    Plasmid

    #153431

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    mScarlet-i

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEBTet-SNAP-CPAP

    Plasmid

    #136815

    Purpose

    Mammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-CENPJ (CENPJ Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV-EF1a-BFPCDK6-W

    Plasmid

    #208756

    Purpose

    Lentiviral vector expressing BFP fused with CDK6 cDNA

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pD649-HAsp-SEMA3C-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156961

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3C (SEMA3C Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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