We narrowed to 24,467 results for: ina
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Plasmid#113441PurposeExpresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cellsDepositorInsertType II-C anti-CRISPR with N-terminal 6xHis and TEV site
Tags6xHisExpressionBacterialPromoterlacIAvailable SinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
UR1Ct (265-1040)
Plasmid#115647Purposeexpression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)DepositorInsertRag1 (265-1040) (Rag1 Mouse)
Tags8XHis-MBP-PreScissionExpressionMammalianMutationtruncated Rag1 (aa 265-1040)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-stop
Plasmid#113745PurposeContains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.DepositorInsertstop cassette
UseCRISPR and Gateway CloningMutationAdded 3 stop codons to the original sequence; see…Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-mCit
Plasmid#113451PurposeLentiviral ProteinA-mCitrine expression vectorDepositorInsertProteinA-mCitrine
UseLentiviralExpressionMammalianPromoterhUBbCAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-NL
Plasmid#113444PurposeProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)DepositorInsertProteinA-mCitrine-NanoLuc
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2162
Plasmid#110136PurposeEpidermal Mitochondrial-tagged Florescent Calcium SensorDepositorInsertmito::GCaMP3
Tagsmito::GCaMP3ExpressionWormPromoterPcol-19Available SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mVenus
Plasmid#105791PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mVenusExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-FLAG
Plasmid#105783PurposeExpression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-FLAGExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZL1-ZF - myelin basic protein a (mbpa)
Plasmid#103007PurposePlasmid template for making in-situ hybridization probe for myelin basic protein a (mbpa) gene zf-fj330a07.y1.DepositorInsertmyelin basic protein (mbpa Zebrafish)
PromoterSp6Available SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 48-232 (#182)
Plasmid#99920Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
TagsHAExpressionMammalianMutationN-terminal deletion; encodes AA 48-232Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHU6_chr12_FAM19A2-down-gRNA-for
Plasmid#81223PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the downstream region of FAM19A2 locusDepositorInserthU6 expression of gRNA
ExpressionMammalianPromoterU6Available SinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
DYRK2_HUMAN_D0
Plasmid#79729PurposeThis plasmid encodes the kinase domain of DYRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
VRK3_HUMAN_D0
Plasmid#79681PurposeThis plasmid encodes the kinase domain of VRK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSK_HUMAN_D0
Plasmid#79683PurposeThis plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_Ch12_62418577-62418652
Plasmid#81212PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
ExpressionMammalianPromoterpCBaAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCALNL_chr13_102010574-102010650
Plasmid#81213PurposepCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)DepositorInsertgix-Neo-gix deletion cassette upstream of EGFP
ExpressionMammalianPromoterpCBaAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_chr12_FAM19A2-up-gRNA-rev
Plasmid#81220PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the upstream region of FAM19A2 locusDepositorInserthU6 expression of gRNA
ExpressionMammalianPromoterU6Available SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB424
Plasmid#68689PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mouse cdk6 D163N
Plasmid#75173PurposeMammalian expression of mouse cdk6 with D163NDepositorAvailable SinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
E0030 (YFP)_CD
Plasmid#66031PurposeMoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]DepositorInsertFluorescent reporter - YFP
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only