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Ssh3 gRNA#1

Plasmid

#163400

Purpose

Cas9-mediated knockout of Ssh3 in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh3 (Ssh3 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GP758

Plasmid

#164541

Purpose

Expresses Sstr3-FLAG-Ub

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Somatostatin receptor type 3 (Sstr3 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

WT

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pZA39

Plasmid

#158517

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA

Tags

6xHA

Expression

Plant

Mutation

L17E

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA30

Plasmid

#158508

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc

Tags

4xMyc

Expression

Plant

Mutation

D481V

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA38

Plasmid

#158516

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA

Tags

6xHA

Expression

Plant

Mutation

D481V

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA46

Plasmid

#158524

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP

Tags

eGFP

Expression

Plant

Mutation

D481V

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA47

Plasmid

#158525

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP

Tags

eGFP

Expression

Plant

Mutation

L17E

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_STS

Plasmid

#139799

Purpose

expresses biosynthetic enzyme STS

Depositor

Roman Jerala

Publication

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

STS (STS Mustard Weed, vitis vinifera)

Expression

Bacterial

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLENTI_Hu_ECT2(T327D)

Plasmid

#136332

Purpose

Lentiviral expression of human ECT2_T327D

Depositor

Guillermo de Cárcer, Marcos Malumbres

Publication

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T327D

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-Mm_MyoGEF(WT)

Plasmid

#136327

Purpose

Mouse MyoGEF-WT in Gateway pENTR

Depositor

Guillermo de Cárcer, Marcos Malumbres

Publication

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Gateway Cloning

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbu

Plasmid

#129637

Purpose

Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

Depositor

Matthias Bochtler

Publication

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbu

Tags

His6-tag

Expression

Bacterial

Mutation

V14A and G107H

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbuT1A

Plasmid

#129638

Purpose

Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

Depositor

Matthias Bochtler

Publication

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbuT1A

Tags

His6-tag

Expression

Bacterial

Mutation

T1A, V14A and G107H

Promoter

T7

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2_K58R_Keima

Plasmid

#127141

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2_Keima (RPS2 Human)

Use

Lentiviral

Mutation

K58R

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2_K275R

Plasmid

#127131

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Mutation

K275R

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-GUCY2C-G549C

Plasmid

#116407

Purpose

Lentiviral expression of GUCY2C G549C

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GUCY2C (GUCY2C Human)

Use

Lentiviral

Mutation

G549C

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJOG587

Plasmid

#105431

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

beta-glucuronidase (2 introns; no ATG-Start codon)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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UKNP1.11.7

Plasmid

#97383

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.21.3

Plasmid

#98201

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.21.1

Plasmid

#98199

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.20.4

Plasmid

#98198

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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