We narrowed to 88,932 results for: Mal

1. pLenti-CMV-TMPRSS2-TwinStrep_Zeo

   Plasmid

   #192000

   Purpose

   Lentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell line

   Depositor

   Jan Carette

   Article

   Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   TMPRSS2

   Expression

   Mammalian

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79

   Plasmid

   #111725

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q79 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBactin-td-mCherry-Cdk5rap2-CTD

   Plasmid

   #196854

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW TetO HA-AMPKa2 WT

   Plasmid

   #69826

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLgw ELK4-V5-EcoDam

   Plasmid

   #98607

   Purpose

   Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK4 (Elk4 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   V5 and Dam (DNA adenine methyltransferase)

   Expression

   Mammalian

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-hDAGLa-V5

   Plasmid

   #87674

   Purpose

   Expresses human DAGLa protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

   Depositor

   Pat Doherty

   Article

   Zhou et al Mol Cell Neurosci. 2016 Oct;76:76-86. do

   Insert

   Human Diacylglycerol Lipase Alpha (DAGLA Human)
   

   Tags

   HA and V5 tag

   Expression

   Mammalian

   Mutation

   Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

   Promoter

   T7

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PEmax-P2A-hP53DD

   Plasmid

   #214084

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PEmax-P2A-hP53DD (TP53 SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. DHC1-AID-Clover donor (Neo)

   Plasmid

   #158621

   Purpose

   Donor plasmid for tagging DHC1 with AID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR; Tagging donor

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti PGK mKate2-OGT 5N5A (Puro)

   Plasmid

   #154293

   Purpose

   Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x FLAG and mKate2

   Expression

   Mammalian

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations; HCF-…

   Promoter

   PGK

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. cmamxA2-GFP

    Plasmid

    #107197

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    cmannexin A2 (ANXA2 Human)
    

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    all type II Ca2+ binding sites deleted ( D161A, E…

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-Sox2-17

    Plasmid

    #193353

    Purpose

    Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-17 (Sox2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    5'LTR

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. shERK2b-mlpx-puro

    Plasmid

    #65230

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBactin-AcGFP-Cdk5rap2-CTD

    Plasmid

    #196853

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX335 HTT sgRNA-a

    Plasmid

    #87201

    Purpose

    pX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTT

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    HTT (HTT Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

    Plasmid

    #156411

    Purpose

    pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4A-WT (KRAS Human)
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 PA-mCit-BAD

    Plasmid

    #113459

    Purpose

    ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 associated agonist of cell death (BAD Human)
    

    Tags

    ProteinA-mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1 KBTBD4 P311PP

    Plasmid

    #184628

    Purpose

    Mammalian expression vector with N-terminal 3xFlag for KBTBD4 P311PP expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 P311PP (KBTBD4 Human)
    

    Expression

    Mammalian

    Mutation

    P311PP

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA 3.1 KBTBD4 R312RG

    Plasmid

    #184630

    Purpose

    Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)
    

    Expression

    Mammalian

    Mutation

    R312RG

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX335 HTT sgRNA-b

    Plasmid

    #87200

    Purpose

    pX335 vector encoding SpCas9n and a chimeric guide RNA targeting 5' UTR of HTT

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    HTT (HTT Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. shERK2a-mlpx-puro

    Plasmid

    #65229

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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