We narrowed to 92,229 results for: lias
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Plasmid#232625PurposeLentiviral plasmid expressing gRNA targeting the enhancer region in Chr20:49345720 (hg38) locus with lentiGuide-Hygro-eGFP (Addgene #99375) as backbone.DepositorInsertS. pyogenes sgRNA cassette
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry
Plasmid#225584PurposeMammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2 (S54L+T127A)-T2A-mCherry
TagsmCherryExpressionMammalianMutationT21R-F8.2 (S54L+T127A)PromoterCMVAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-LAMP1-Y414A
Plasmid#222319PurposeSynchronize the trafficking of LAMP1-Y414A from the ER.DepositorInsertStreptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)
ExpressionMammalianMutationTyr414 to AlaPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-LAMP1-Y414A
Plasmid#222320PurposeSynchronize the trafficking of LAMP1-A17 from the ER.DepositorInsertStreptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pScalps-Puro-FLAG_HA-Regnase-1 D141N
Plasmid#222663PurposeLentiviral vector to express mouse Regnase-1 D141N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12a (Zc3h12a Mouse)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationmutated aspartic acid 141 to asparagine to abroga…PromoterSFFVAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-NRG1-ScNeo
Plasmid#209911PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-chimpanzee cGAS-HA
Plasmid#186873PurposeDoxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transductionDepositorInsertChimpanzee cGAS with C-terminal HA tag (CGAS Pan troglodytes)
UseRetroviralTagsHAPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-mouse cGASdeltaN-HA
Plasmid#186876PurposeDoxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transductionDepositorInsertMouse cGAS truncation mutant (148-508 a.a.) with C-terminal HA tag (Cgas Synthetic, Mouse)
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-gibbon cGASdeltaN-HA
Plasmid#186861PurposeDoxycycline-dependent expression of gibbon cGAS gene in mammalian cells by retroviral transductionDepositorInsertGibbon cGAS trucation mutant (152-514 a.a.) with C-terminal HA tag (CGAS Synthetic, Hylobatidae)
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-rhesus macaque cGAS-HA
Plasmid#186865PurposeDoxycycline-dependent expression of rhesus macaque cGAS gene in mammalian cells by retroviral transductionDepositorInsertRhesus macaque cGAS with C-terminal HA tag (CGAS Macaca mulatta)
UseRetroviralTagsHAPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-orangutan cGAS-HA
Plasmid#186871PurposeDoxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transductionDepositorInsertOrangutan cGAS with C-terminal HA tag (CGAS Pongo abelii)
UseRetroviralTagsHAPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-human cGAS-GFP
Plasmid#186844PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-HA-human cGAS
Plasmid#186851PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScalps-Puro-FLAG-HA-Regnase-3 D252N
Plasmid#222664PurposeLentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationmutated aspartic acid 252 to asparagine to abroga…PromoterSFFVAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
gamma-tubulin (1-335)
Plasmid#226519PurposeExpressing a fragment of gamma-tubulin containing gamma-tubulin GTPase domainDepositorInsertTUBG1 N-terminal amino acids 1-335 (TUBG1 Human)
UsePcdna3.1ExpressionMammalianMutationDeleted amino acids 334-451 and introduced the fo…Available SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Regnase-3 D252N-T2A-copGFP
Plasmid#222661PurposeLentiviral vector to express mouse Regnase-3 D252NDepositorInsertZc3h12c (Zc3h12c Mouse)
UseLentiviralExpressionMammalianMutationmutated aspartic acid 252 to asparagine to abroga…PromoterEF1αAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CTLA-4-GCN4-His6
Plasmid#223582PurposeProtein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGECPL.BC10.MG
Plasmid#223822PurposeContains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.DepositorInsertEf1a-Driven Cre-P2A-FLuc
UseCRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…TagsP2AExpressionMammalianPromoterEf1aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT-cbm_PCaspase
Plasmid#214047PurposeBacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
ExpressionBacterialMutationK121E, Q122A, N274A, R276E, Y285A, F286APromoterlacUV5 promoterAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT_PCaspase-N-C-term
Plasmid#214046PurposeBacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
ExpressionBacterialMutationaa 1-153, 154-666PromoterlacUV5 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only