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pME-3x-FLAG-Ubiquitin (JDW 1098)

Plasmid

#224513

Purpose

A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3x-FLAG-Ubiquitin

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DNM2-R465W

Plasmid

#155262

Purpose

Mammalian expression of rat DNM2 fused to HA

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

dynamin 2 (Dnm2 Rat)

Tags

HA

Expression

Mammalian

Mutation

Arginine 465 to Tryptophan

Available Since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-mmCdh5pro (JDW 1239)

Plasmid

#224478

Purpose

Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Cdh5 promoter

Expression

Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DNM2-W525L

Plasmid

#155263

Purpose

Mammalian expression of rat DNM2 fused to HA

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

dynamin 2 (Dnm2 Rat)

Tags

HA

Expression

Mammalian

Mutation

Tryptophan 525 to Leucine

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pME-MCS (JDW 455)

Plasmid

#229832

Purpose

A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attL1/L2 flanked MCS

Use

Gateway Cloning

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5

Plasmid

#81062

Purpose

Gateway entry vector encoding full length UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)

Use

Cloning

Mutation

K503R

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-BLM

Plasmid

#80070

Purpose

Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

Depositor

Nathan Ellis

Article

Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

Insert

BLM (BLM Human)

Tags

GFP

Expression

Mammalian

Mutation

If correct ID, the cDNA is from HeLa and has char…

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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eDrEGFR

Plasmid

#184000

Purpose

Optically controlled eDrEGFR expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrEGFR (EGFR Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a (JDW 1164)

Plasmid

#224473

Purpose

Gateway compatible 5' entry clone containing the human EF1a promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF1a promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-Lb

Plasmid

#138096

Purpose

Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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