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pCambia1300-ba-ar

Plasmid

#226815

Purpose

Efficient screening of gene editing in plants (Basta resistance).

Depositor

Daisuke Miki

Article

Kong et al Hortic Res. 2025 Nov 5;12(11):uhaf196. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSIn-H3K9ac BS

Plasmid

#235131

Purpose

H3K9ac FRET biosensor for real-time, single-cell monitoring of H3K9ac levels

Depositor

Qin Peng

Article

Li et al Med-X. 2024; 2, 26. doi: 10.1007/s44258-

Insert

BD1-Ypet-34mer-ECFP-H3

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSIn-H3K9ac Y97F BS

Plasmid

#235132

Purpose

Mutant (negative control) Y97F biosensor for pSin-H3K9ac Full

Depositor

Qin Peng

Article

Li et al Med-X. 2024; 2, 26. doi: 10.1007/s44258-

Insert

BD1Y97F-Ypet-34mer-ECFP-H3

Use

Lentiviral

Expression

Mammalian

Mutation

BD1Y97F

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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VN-dCasE-VC-GK

Plasmid

#164277

Purpose

Express VN-dCasE-VC protein in mammalian cells. This plasmid is also referred to as as VN-dEcCas6-VC-GK in the publication.

Depositor

Chunyu Han

Article

Gao et al Nucleic Acids Res. 2022 May 6;50(8):e46.

Insert

VN-dCasE-VC

Expression

Mammalian

Mutation

H20A in EcCasE

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLPo-3xmiR122-WPRE-HGHpA

Plasmid

#220938

Purpose

FLPo recombinase

Depositor

Chenghua Gu

Article

Krolak et al Cell. 2025 Jul 11:S0092-8674(25)00732-9.

Insert

Codon-optimized FLPe recombinase

Use

AAV

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5425

Plasmid

#229032

Purpose

Expression vector for 10xHis-phi29 DNA polymerase (catalytically inactive mutant D249E): PT7-lacO-g10leader-p2 dead-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP (D249E, catalytically inactive)

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SV40-puro-EF1a-HA-NLGN1dAChE-bc

Plasmid

#220437

Purpose

Expresses HA-tagged human NLGN1 with K578A/V579A substitutions

Depositor

Paul Blainey

Article

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Insert

Neuroligin-1 (NLGN1 Human)

Use

Lentiviral

Tags

HA-tag

Expression

Mammalian

Mutation

K578A and V579A substitutions

Promoter

EF1a

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crF1

Plasmid

#213046

Purpose

Guide RNA expression vector with the synthetic crRNA crF1 targeting the SWITCHER construct with the target site TF1

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crF1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crR2

Plasmid

#213047

Purpose

Guide RNA expression vector with the synthetic crRNA crR2 targeting the SWITCHER construct with the target site TR2

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crR2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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POC1426

Plasmid

#221573

Purpose

Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1427

Plasmid

#221574

Purpose

Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB027

Plasmid

#234636

Purpose

pET-28a(+) based plasmid for expression of H16_B1102 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1102 (H16_RS24270 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB028

Plasmid

#234637

Purpose

pET-28a(+) based plasmid for expression of H16_B1148 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1148 (H16_RS24495 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB029

Plasmid

#234638

Purpose

pET-28a(+) based plasmid for expression of H16_B1264 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1264 (H16_RS25065 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB030

Plasmid

#234639

Purpose

pET-28a(+) based plasmid for expression of H16_B1335 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1335 (H16_RS25400 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB024

Plasmid

#234633

Purpose

pET-28a(+) based plasmid for expression of H16_A0285 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A0285 (H16_RS01400 Cupriavidus necator H16)

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB026

Plasmid

#234635

Purpose

pET-28a(+) based plasmid for expression of H16_A3514 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A3514 (H16_RS17530 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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