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pBullet-cyt-n

Plasmid

#53065

Purpose

destination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-tgn-n

Plasmid

#53071

Purpose

destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A-miR-375-mutE

Plasmid

#53710

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site E (refer to cita…

Available Since

July 11, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-stop-mut-miR-375-mutA-E

Plasmid

#53714

Purpose

Luciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutations

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on the stop codon of firefly luciferase …

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA&B

Plasmid

#53711

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A&B (refer t…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutD

Plasmid

#53709

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site D (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutC

Plasmid

#53708

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site C (refer to cita…

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutB

Plasmid

#53707

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site B (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pMiR-CIP2A-miR-375-mutA

Plasmid

#53706

Purpose

Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site A (refer to cita…

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pCR4-*mRFP

Plasmid

#47118

Purpose

Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

*mRFP

Expression

Bacterial

Mutation

No start codon

Promoter

Lac

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag GK2

Plasmid

#20494

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

GK2 (GK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag HSCB

Plasmid

#20472

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HSCB (HSCB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag DAK

Plasmid

#20473

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

DAK (DAK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

Plasmid

#101297

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-906 of alpha-Catenin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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HCT55_rAAV-eHGT453m_eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA

Plasmid

#223945

Purpose

Direct-expressing SYFP2 AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

SYFP2

Use

AAV

Mutation

N/A

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCS-APTA

Plasmid

#247077

Purpose

pCS27 containing aroL, ppsA, tktA, aroG(fbr) from E. coli

Depositor

Yajun Yan

Article

Shen et al Metab Eng. 2017 Jul;42:52-58. doi: 10.10

Inserts

aroL
ppsA
tktA
aroG(fbr)

Expression

Bacterial

Mutation

feedback-inhibition-resistant

Promoter

PLlacO1

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT81

Plasmid

#218732

Purpose

Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

Insert

IFT81 (IFT81 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-NKX2.2

Plasmid

#134670

Purpose

Retroviral expression of mouse Nkx2.2

Depositor

Marius Wernig

Article

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Retroviral

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mCherry-CyrCR1.mutant

Plasmid

#128746

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)

Expression

Mammalian

Mutation

5 point mutations disrupting the miR-7 site

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGP8A-ccdB

Plasmid

#83883

Purpose

Destination vector for integration at HIS3, expression from the ADH1 promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway Cloning

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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