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18065-M04-412

Plasmid

#225667

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

Plasmid

#224496

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP-P2A-HA-iRFP-caax

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV(T9)-DERA-sgresis

Plasmid

#222622

Purpose

Lentiviral vector that expresses sgRNA resistant DERA in mammalian cells

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

DERA (DERA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-EGFP-GNB1L

Plasmid

#222621

Purpose

Mammalian expression vector of flag-tagged GNB1L

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

GNB1L (GNB1L Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLX304-BCL-XL-499/500R

Plasmid

#203575

Purpose

Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

silent point mutations to make resistant shRNA 49…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

Plasmid

#201821

Purpose

An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

hChR2(H134R)-V5-ER-LBD (ChRERa)

Use

AAV

Promoter

SYN1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-HA-Dre]-lox-Akna-mOrange2

Plasmid

#196893

Purpose

Neuron-specific expression of Akna fused to mOrange2. Used in combination with Talpha1-iCre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akna-mOrange2 (Akna Mouse)

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-Akna-mOrange2

Plasmid

#196880

Purpose

Neuron-specific expression of the centrosomal protein Akna fused to mOrange2

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akna-mOrange2 (Akna Mouse)

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SMSr-TEV-Twin-Strep

Plasmid

#202530

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Insert

SAMD8 (SAMD8 Human)

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

deleted stop codon (TGA)

Promoter

CAG and chicken β-actin promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

Plasmid

#197454

Purpose

The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 Delta-WD40 propeller (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW& dimerization domain deleted.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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