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FLAG-SLC30A10-E25A

Plasmid

#82345

Purpose

Expresses FLAG-tagged SLC30A10-E25A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

E25A

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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mCitrine-USP19 1-1290

Plasmid

#78595

Purpose

Express mCitrine-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 1-1290 (USP19 Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
GFP-Drosha1-390

Plasmid

#62521

Purpose

C-terminal aa391-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 391-1374 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 391-1374

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pAV106-pTDH3-mCherry-10xSumo-6xSIM

Plasmid

#116928

Purpose

Yeast integrative vector for expression of the SUMO-SIM complex

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10xSUMO-6xSIM

Tags

mCherry

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCALNL-Kir2.1

Plasmid

#125578

Purpose

Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB3020(gRNA X-2)

Plasmid

#73282

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3045(gRNA XI-3)

Plasmid

#73287

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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mIFP-Vimentin-7

Plasmid

#56242

Purpose

Localization: Intermediate Filaments, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Vimentin (VIM Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP24-LaminA-C-18

Plasmid

#56267

Purpose

Localization: Nuclear Envelope, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LaminA (LMNA Human)

Tags

mIFP24

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Nup50-N-10

Plasmid

#56233

Purpose

Localization: Nuclear Pore, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Nup50 (NUP50 Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-CD81-10

Plasmid

#56248

Purpose

Localization: Tetraspanin/Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CD81 (CD81 Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEGFP-TRAF4

Plasmid

#58318

Purpose

EGFP-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pYPQ145-ZmUbi-tRNA

Plasmid

#158403

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-myc

Plasmid

#109422

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope

Tags

BAP, HA, His6, T7, and myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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fIM1266

Plasmid

#132578

Purpose

pZE1-pLsoxS-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

pLsoxS-mCherry-TermT1

Expression

Bacterial

Promoter

pLsoxS

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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TJF117

Plasmid

#132572

Purpose

pZE1-oxySp-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

oxySp-mCherry-TermT1

Expression

Bacterial

Promoter

oxySp

Available Since

Aug. 13, 2022

Availability

Academic Institutions and Nonprofits only

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TJF64

Plasmid

#132573

Purpose

pZE1-katGp-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

katGp-mCherry-TermT1

Expression

Bacterial

Promoter

katGp

Available Since

Aug. 13, 2022

Availability

Academic Institutions and Nonprofits only

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TJF66

Plasmid

#132574

Purpose

pZE1-ahpCp-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

ahpCp-mCherry-TermT1

Expression

Bacterial

Promoter

ahpCp

Available Since

Aug. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB

Plasmid

#199305

Purpose

pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

CjPglB with flag tag

Tags

Flag tag

Expression

Bacterial

Promoter

pBAD

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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ZO-1 shRNA 2072

Plasmid

#37207

Depositor

James Anderson, Alan Fanning

Article

Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

Insert

dog ZO-1 shRNA (TJP1 canine)

Use

RNAi

Expression

Mammalian

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only