We narrowed to 154 results for: SPT

1. pS238D1::sptse5-CT_phoA-lacZalpha

   Plasmid

   #192961

   Purpose

   Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT_phoA-lacZ_
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1317) fused to phoA-lacZal…

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKTop::sptse5-CT (1169-1281)

   Plasmid

   #192950

   Purpose

   For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1281)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1281)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKTop::sptse5-CT (1169-1269)

   Plasmid

   #192949

   Purpose

   For membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1269)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for tse5 (1169-1269)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKTop::sptse5-CT (1169-1300)

   Plasmid

   #192951

   Purpose

   For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   sptse5-CT (1169-1300)
   

   Use

   Tags

   PelB signal peptide

   Expression

   Bacterial

   Mutation

   encodes for sptse5 (1169-1300)

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CPH3566 (pDEST15_Spt6 1223-1451)

   Plasmid

   #91814

   Purpose

   Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deleted residues 1-1222

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CPH1141 (pET151_Spt6 1247-1451)

   Plasmid

   #91815

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   deleted residues 1-1246

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDSM-cd-Psptdh3-ACO-Tagtef1

   Plasmid

   #127734

   Purpose

   Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   aconitase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Psptdh3

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. CPH3567 (pET151_Spt6 1247-1451 R1282H)

   Plasmid

   #91816

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed arginine 1282 to histidine; deleted resid…

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CPH3495 (pET151_Spt6 1247-1451 K1435A)

    Plasmid

    #91817

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 1435 to alanine; deleted residues …

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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