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Plasmid#112493PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationdeletion of C terminus (AA290-671 deleted)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-delDD
Plasmid#112494PurposeMammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationdeletion of DD domain (AA584-671 deleted)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53_1-87-mCer
Plasmid#211441Purposemammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mCeruleanDepositorInsertC17orf53 (HROB Human)
UseTagsmCeruleanExpressionMammalianMutationDeletion of aa 88-646PromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53_1-87-mVen
Plasmid#211442Purposemammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mVenusDepositorInsertC17orf53 (HROB Human)
UseTagsmVenusExpressionMammalianMutationDeletion of aa 88-646PromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_NSlmb-vhhGFP4
Plasmid#35579DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF125 Ring Mutant
Plasmid#122046Purposeexpress RNF125 RMDepositorInsertRNF125 RM (RNF125 Human)
UseTagsFlagExpressionMammalianMutationRing mutant C37A, C40APromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1
Plasmid#145148PurposeMammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Fli1
Plasmid#66976PurposeExpression plasmid for mouse Fli1; adds Flag tag to the N-terminus of proteinDepositorInsertFli1 (Fli1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_NSnoFbox-vhhGFP4
Plasmid#35580DepositorInsertNSnoFbox-vhhGFP4 (slmb Fly)
UseTagsExpressionMammalianMutationF box domain of slmb deleted.PromoterAvailable sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ShhN-Ren
Plasmid#37677DepositorInsertshh (Shh Mouse)
UseTagsrenilla luciferaseExpressionMammalianMutationsoluble form (see comments)PromoterCMVAvailable sinceNov. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala
Plasmid#208172PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
UseTagsStrep-tagExpressionMammalianMutationArg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala
Plasmid#208173PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
UseTagsStrep-tagExpressionMammalianMutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala
Plasmid#208174PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)DepositorInsertN-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
UseTagsStrep-tagExpressionMammalianMutationLys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPE1-del163-278_Z
Plasmid#148117PurposeMammalian Expression of HshnRNPE1-del163-278DepositorInsertHshnRNPE1-del163-278 (PCBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53_1-241-mCer
Plasmid#211443Purposemammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mCeruleanDepositorInsertC17orf53 (HROB Human)
UseTagsmCeruleanExpressionMammalianMutationDeletion of aa 242-646PromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53_1-241-mVen
Plasmid#211444Purposemammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mVenusDepositorInsertC17orf53 (HROB Human)
UseTagsmVenusExpressionMammalianMutationDeletion of aa 242-646PromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcDNA3
Plasmid#18964DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-LIN-29-pcDNA3.1-
Plasmid#52515Purposeexpresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertLin-29 b (lin-29 Nematode)
UseTagsRGS-6xHisExpressionMammalianMutationwt (changed coding sequence in 5' (5th codon…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 5HT2A_NB2_1
Plasmid#127690PurposePlasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.DepositorInsert5HT2A_NB2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Myb
Plasmid#66980PurposeExpression plasmid for mouse Myb; adds Flag tag to the N-terminus of proteinDepositorInsertMyb (Myb Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CTE-Luc
Plasmid#60881PurposeReporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTEDepositorInsertLuc-CTE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-H2-18 Light chain
Plasmid#216308PurposeExpresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 FabDepositorInsertH2-18 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA
Plasmid#235678PurposePlasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆CDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1neo-NLucYAP15
Plasmid#107610Purposemeasure LATS activity by Luciferase AssayDepositorInsertNLucYAP15
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MvChR1_EYFP_pcDNA3.1
Plasmid#159119PurposeThis plasmid encodes MvChR1 rhodopsin domainDepositorInsertMvChR1
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras light chain
Plasmid#216294PurposeExpresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras FabDepositorInsertTras light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Pert Light chain
Plasmid#216310PurposeExpresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert FabDepositorInsertPert Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Light chain
Plasmid#216297PurposeExpresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S FabDepositorInsert39S Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Light chain
Plasmid#216304PurposeExpresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1neo-14-3-3-CLuc
Plasmid#107611Purposemeasure LATS activity by Luciferase AssayDepositorInsertpCDNA3.1hygro-BamHI-CLuc1433-NotI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only