Addgene

We narrowed to 128 results for: ZsGreen

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p23-mNeongreen-DDX21 DAT-mRuby3

Plasmid

#179226

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Use

Tags

mNeongreen and mRuby3

Expression

Mammalian

Mutation

G234D, K235A

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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p23-mNeongreen-DDX21 HGV-mRuby3

Plasmid

#179227

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Use

Tags

mNeongreen and mRuby3

Expression

Mammalian

Mutation

D338H, E339G

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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p17xQUAS-Luciferase, α-cry:mCherry

Plasmid

#180482

Purpose

Inducible gene expression vector p17xQUAS-Luciferase, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Inserts

Luciferase
17x QUAS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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p23-mNeongreen-DDX21 HGV

Plasmid

#179230

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Use

Tags

mNeongreen

Expression

Mammalian

Mutation

D338H, E339G

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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p23-mNeongreen-DDX21 DAT

Plasmid

#179229

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Use

Tags

mNeongreen

Expression

Mammalian

Mutation

G234D, K235A

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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p23-mRuby3-DDX21 HGV

Plasmid

#179233

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

D338H, E339G

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-Bi-β-chain

Plasmid

#133731

Purpose

Expresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Inserts

b-Chain_gePSI
ZsGreen1

Use

Tags

Expression

Mammalian

Mutation

Only one part of the gePSI-system (startcodon + a…

Promoter

pTRE3G-Bi

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE3G-Bi-α-chain

Plasmid

#133730

Purpose

Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Inserts

a-Chain_gePSI
ZsGreen1

Use

Tags

Expression

Mammalian

Mutation

Only one part of the gePSI-system (aa1-141 + stop…

Promoter

pTRE3G-Bi

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

Plasmid

#194755

Purpose

Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

Plasmid

#194753

Purpose

Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194752

Purpose

Lentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-VP64-dCas9-VP64-ZsG

Plasmid

#192668

Purpose

Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1

Depositor

Jeffrey Miner

Insert

VP64-dCas9-VP64, ZsGreen1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

TRE3G

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

Plasmid

#192667

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

Depositor

Jeffrey Miner

Insert

VP64-dCas9-VP64, ZsGreen1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-dCas9-VP64-MS2-VP64-ZsG

Plasmid

#192669

Purpose

Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

Depositor

Jeffrey Miner

Insert

dCas9-VP64, MS2-VP64, ZsGreen1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

TRE3G

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

Plasmid

#192665

Purpose

3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

Depositor

Jeffrey Miner

Insert

dCas9-VP64, MS2-VP64, ZsGreen1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM230-WT-IRES(isoform 2)

Plasmid

#99564

Purpose

mammalian expression of TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM230-WT-IRES(isoform 1)

Plasmid

#99558

Purpose

mammalian expression of TMEM230 (isoform 1) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM230-M4/X184PG-IRES(isoform 2)

Plasmid

#99568

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

M4/X184PG

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM230-M3/Y92C-IRES(isoform 2)

Plasmid

#99567

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

M3/Y92C

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM230-M2/X184W-IRES(isoform 2)

Plasmid

#99566

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

M2/X184W

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge