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Showing: 101 - 115 of 115 results

  1. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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  2. DT034A

    Plasmid
    #159395
    Purpose
    Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
    Use
    Synthetic Biology
    Tags
    FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…
    Expression
    Bacterial
    Mutation
    Promoter
    J23117 and pLux promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    159395-plasmid-map-sequence-id-393061

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  3. pDUBI

    Plasmid
    #179274
    Purpose
    Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d
    Inserts
    cellodextrin phosphorylase
    cellobiose phosphorylase
    C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
    Use
    Synthetic Biology
    Tags
    6xHIS and Strep-Tag II
    Expression
    Bacterial
    Mutation
    sucrose phosphorylase
    Promoter
    T7 lacO
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179274-plasmid-map-sequence-id-358736

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  6. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid
    #60866
    Purpose
    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    CohIII
    CBM
    Use
    Tags
    6xHIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    Cysteine 63 to Serine in CBM module
    Promoter
    T7
    Available since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60866-plasmid-map-sequence-id-98102

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  7. pAP84

    Plasmid
    #86554
    Purpose
    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile
    Depositor
    Wiep Klaas Smits
    Insert
    Pveg-sLucopt
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86554-plasmid-map-sequence-id-173277

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  10. pJBL6392

    Plasmid
    #193141
    Purpose
    Synthetic OFF-logic Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter
    Depositor
    Julius Lucks
    Article
    Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d
    Insert
    Synthetic flipped logic Cbe riboswitch with sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    193141-plasmid-map-sequence-id-379071

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  11. pJK02

    Plasmid
    #105133
    Purpose
    Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT
    Depositor
    Joseph Sorg
    Article
    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.
    Inserts
    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    105133-plasmid-map-sequence-id-202234

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  12. pKM126

    Plasmid
    #105134
    Purpose
    Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT
    Depositor
    Joseph Sorg
    Article
    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.
    Inserts
    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    105134-plasmid-map-sequence-id-199249

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  13. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

    Plasmid
    #129105
    Purpose
    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production
    Depositor
    Eleftherios Terry Papoutsakis
    Article
    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10
    Insert
    BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129105-plasmid-map-sequence-id-255085

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  14. pUC19-B1UZL4

    Plasmid
    #103085
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B1UZL4(Cas9 coding gene from Clostridium perfringens D str. JGS1721)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103085-plasmid-map-sequence-id-201733

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  15. pUC19-B8I085

    Plasmid
    #103089
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B8I085(Cas9 coding gene from Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103089-plasmid-map-sequence-id-201739

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Showing: 101 - 115 of 115 results