We narrowed to 409 results for: ta cloning vector

1. pDONR223-TAF5-FL_WobbleMut

   Plasmid

   #209053

   Purpose

   Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 full length
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-TAF5-∆ex8_WobbleMut

   Plasmid

   #209054

   Purpose

   Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 deltaexon-8
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-TAF5-∆ex8

   Plasmid

   #209048

   Purpose

   Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 deltaexon-8
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

   Plasmid

   #199585

   Purpose

   Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
   AkaLuc
   Blasticidin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

   Plasmid

   #65662

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   introduced HindIII site (final goal to exchange F…

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBDP

   Plasmid

   #217827

   Purpose

   Stuffer plasmid with golden gate compatible bidirectional promoter

   Depositor

   Timothy Whitehead

   Article

   Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

   Insert

   Gal1/Gal10 Bidirectional Promoter
   

   Use

   Cloning vector

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_tKiMBImut-T2A-caMEK

   Plasmid

   #199579

   Purpose

   Express tKiMBImut(AA) and caMEK in an AAV vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
   constitutively active MEK
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A

   Plasmid

   #65660

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed glutamic acid 97 to alanine, lysine 100 t…

   Promoter

   CMV, 2x Tet

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXeDNA3.1-LacZ

   Plasmid

   #242746

   Purpose

   pXeDNA3-LacZ TA-cloning vector for dual expression: CMV enhancer/promoter in mammalian cells and Xenopus β-globin 5'/3'UTRs in oocytes; BGHpA, AmpR, NeoR, lacZα for blue-white screening.

   Depositor

   Luis Yanez-Guerra

   Type

   Empty backbone

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJS646

    Plasmid

    #183175

    Purpose

    Y2H vector compatible with pJS647 PYR1 insert

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR4 PARP2 E614Q

    Plasmid

    #192545

    Purpose

    pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
    

    Use

    Gateway entry vector

    Mutation

    changed Glu 614 to Gln

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT-TODestFLAGHA_RBPMS_KDR_111del

    Plasmid

    #65659

    Purpose

    FLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced stop codon such as to produce deletion…

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23119

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT-TODestFLAGHA_RBPMS2

    Plasmid

    #59390

    Purpose

    Destination vector with RBPMS2 for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONOR223 RpH-LAMP1-3xFLAG

    Plasmid

    #163019

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    RpH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONOR223 R2pH-LAMP1-3xFLAG

    Plasmid

    #157941

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG, mCherry, and pHluorin

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR™221_RBPMS2

    Plasmid

    #59389

    Purpose

    pDONR vector with RBPMS2

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Promoter

    T7

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas-pML-1B_6xHis-Cas12m

    Plasmid

    #192282

    Purpose

    MmCas12m fused to His-tag for protein purification

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12m
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CD44P pGL3

    Plasmid

    #19122

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 promoter (CD44 Human)
    

    Use

    Non viral cloning vector

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-UltraCas12a-6xNLS

    Plasmid

    #209023

    Purpose

    Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    UltraCas12a-6xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    M537R/F870L

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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