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pSB116 - pL2_pSB90_fLUC-I_P19

Plasmid

#123191

Purpose

binary plant vector for transient TBSV P19 and fLUC (with intron) expression

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::P19::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB118 - pL2_pSB90_fLUC-I_HcPro

Plasmid

#123193

Purpose

binary plant vector for transient TEV HcPro and fLUC (with intron) expression

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBabepuro3-p18Flag

Plasmid

#24933

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

P18 (CDKN2C Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 proHB-EGF NC

Plasmid

#11605

Depositor

Rosalyn Adam

Article

Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

Insert

HB-EGF (HBEGF Human)

Use

Tags

Expression

Mammalian

Mutation

Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
tet H-ras val12

Plasmid

#8900

Depositor

Lynda Chin

Article

Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

Insert

ras val12 (HRAS Human)

Use

Tags

Tet-op promoter

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Jan. 13, 2006

Availability

Academic Institutions and Nonprofits only
pBabepuro3-p18

Plasmid

#24935

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p18 (CDKN2C Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pSB117 - pL2_pSB90_fLUC-I_V2

Plasmid

#123192

Purpose

binary plant vector for transient TYLCV V2 and fLUC (with intron) expression

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::V2::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB115 - pL2_pSB90_fLUC-I_tGFP-I

Plasmid

#123190

Purpose

binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR_RAF1_94

Plasmid

#111826

Purpose

Knockout Raf1

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting RAF1 94-114 (RAF1 Human)

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

Plasmid

#123188

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLC025 - pL0_StopCodon (CT)

Plasmid

#203905

Purpose

MoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

Stop codon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN381 - pTRV2-CHLH

Plasmid

#203886

Purpose

Viral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CHLH fragment

Use

RNAi

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1243 - pL0_pT3O (pro + 5U)

Plasmid

#203891

Purpose

T3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T3O promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1198 - pL0_pD4H (pro + 5U)

Plasmid

#203894

Purpose

D4H promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

D4H promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSB153 - pL2_pSB90_2x35S::ZCT1::tMAS

Plasmid

#123195

Purpose

binary plant vector for transient expression of ZCT1 from Catharanthus roseus

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::ZCT1::tMAS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB160 - pL2_pSB90_2x35S::ORCA3::tMAS

Plasmid

#123196

Purpose

binary plant vector for transient expression of ORCA3 from Catharanthus roseus

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::ORCA3::tMAS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS

Plasmid

#123199

Purpose

binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

tMAS::rLUC-I::pMAS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB62 - pL0_STR (pro + 5U)

Plasmid

#123186

Purpose

Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

Catharanthus roseus STR1 promoter and 5'UTR

Use

Part for plant expression

Tags

Expression

Plant

Mutation

No BpiI and BsaI sites

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-shC

Plasmid

#21963

Depositor

Lynda Chin

Article

Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

Insert

si NEDD9 (NEDD9 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-HSF1

Plasmid

#31210

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

HSF1 (HSF1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only