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Plasmid#60990PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound dTomatoDepositorInsertdTomato
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-hG
Plasmid#60994PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear EGFPDepositorInsertEGFP
UseZebrafishTagshistone 2B tagExpressionMutationPromoterBactin2Available sinceJan. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-hC
Plasmid#60993PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear ceruleanDepositorInsertCerulean
UseZebrafishTagshistone 2B tagExpressionMutationPromoterBactin2Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-hfR
Plasmid#60998PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear mKate2.DepositorInsertmKate2
UseZebrafishTagshistone 2B tagExpressionMutationPromoterBactin2Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mB
Plasmid#60985PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EBFP2.DepositorInsertEBFP2
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mO
Plasmid#60989PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKODepositorInsertmKO
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mY
Plasmid#60988PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound citrineDepositorInsertcitrine
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-hO
Plasmid#60996PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear mKODepositorInsertmKO
UseZebrafishTagshistone 2B tagExpressionMutationPromoterBactin2Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mC
Plasmid#60986PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound ceruleanDepositorInsertCerulean
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pst39-NST-clone14
Plasmid#133421PurposeS. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).DepositorUseTagsExpressionBacterialMutationORF for Nip1 encodes aa 4-156 (not complete ORF),…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
ptRNA100
Plasmid#154133PurposeExpresses a set of tRNAs in bacterial cellsDepositorInserttRNAs operon under control of Ptac promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkH
Plasmid#182291Purposeexpresses c-di-GMP biosensor in E. coliDepositorInsertmVenusNB-MrkH
UseTagsmVenusNB internal fusion to MrkHExpressionBacterialMutationPromoterproCAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Tt
Plasmid#154124PurposeExpresses wt Ribo-T rRNA and a set of tRNAs in bacterial cellsDepositorInsertsengineered bacterial rRNAs operon under control of pL promotor
bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtac and pLAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN1_EF1a-tNGFR-2A-Puro
Plasmid#200553PurposeDual barcoding cassettes for SSN-seq; Barcode Set 1DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN2_EF1a-tNGFR-2A-Puro
Plasmid#201112PurposeDual barcoding cassettes for SSN-seq; Barcode Set 2DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN3_EF1a-tNGFR-2A-Puro
Plasmid#201113PurposeDual barcoding cassettes for SSN-seq; Barcode Set 3DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN4_EF1a-tNGFR-2A-Puro
Plasmid#201114PurposeDual barcoding cassettes for SSN-seq; Barcode Set 4DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN5_EF1a-tNGFR-2A-Puro
Plasmid#201115PurposeDual barcoding cassettes for SSN-seq; Barcode Set 5DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN6_EF1a-tNGFR-2A-Puro
Plasmid#201116PurposeDual barcoding cassettes for SSN-seq; Barcode Set 6DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN7_EF1a-tNGFR-2A-Puro
Plasmid#201117PurposeDual barcoding cassettes for SSN-seq; Barcode Set 7DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only