Skip to main content
Addgene
Showing: 2951 - 3000 of 4301 results

  3. pET28a Gx-NL2

    Plasmid
    #169107
    Purpose
    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169107-plasmid-map-sequence-id-334716

    Enlarge

  5. pTS2311-Tier1-spacer-PPGK-NLuc_TB

    Plasmid
    #169552
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-NanoLuc Luciferase - mTagBFP fusion
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169552-plasmid-map-sequence-id-364979

    Enlarge

  8. pmirGLO-Smad5-1719

    Plasmid
    #49382
    Purpose
    Insert is a fragment of the Smad5 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.
    Depositor
    Martine Roussel
    Article
    Murphy et al Cancer Res. 2013 Oct 21.
    Insert
    Smad5 (Smad5 Mouse, Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Human phosphoglycerate kinase (PGK) promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    49382-plasmid-map-sequence-id-73405

    Enlarge

  13. p.UTA.2.0-GL-2

    Plasmid
    #82440
    Purpose
    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2
    Depositor
    Jens Gruber
    Article
    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10
    Insert
    Luciferase
    Use
    Tags
    Expression
    Mutation
    The complementary region for GL2 siRNA control wa…
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82440-plasmid-map-sequence-id-168133

    Enlarge

  15. pOPTXcGMPRELUC

    Plasmid
    #68503
    Purpose
    cyclic nucleotide reporter for bacteria or plant cells
    Depositor
    Helen Irving
    Article
    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1
    Insert
    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    Use
    Tags
    luciferase
    Expression
    Bacterial and Plant
    Mutation
    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…
    Promoter
    OPTX promoter with cGMPRE
    Available since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  28. pOPTXGARE

    Plasmid
    #68542
    Purpose
    cyclic nucleotide reporter for bacteria or plant cells
    Depositor
    Helen Irving
    Article
    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1
    Insert
    OPTX promoter with GARE (AT1G33440 Mustard Weed)
    Use
    Tags
    luciferase
    Expression
    Bacterial and Plant
    Mutation
    5x GARE inserted 190 bp 5' pf ATG (GARE = ta…
    Promoter
    OPTX promoter with GARE
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68542-plasmid-map-sequence-id-121994

    Enlarge

Showing: 2951 - 3000 of 4301 results