We narrowed to 9,223 results for: Coli

1. pRAR041

   Plasmid

   #185027

   Purpose

   400 nt l31 promoter region, l31 5'UTR with nt. 47-54 & 76-86 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Nucleotides 47-54 & 76-86 deleted from rpmE …

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCapVQ329R

   Plasmid

   #183029

   Purpose

   the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28

   Depositor

   Ute Romling

   Article

   Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

   Insert

   patatin-like phospholipase variant CapVQ329R
   

   Use

   Tags

   No

   Expression

   Bacterial

   Mutation

   glutamine 329 changed to arginine

   Promoter

   pBAD promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21a Ec-iPGM-bio

   Plasmid

   #162575

   Purpose

   Expresses E. coli iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   E. coli iPGM bio-6His
   

   Use

   Tags

   6His tag, T7 tag, biotinylation sequence, and thr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSP019

   Plasmid

   #168195

   Purpose

   expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.

   Depositor

   James Fleckenstein

   Insert

   eatA[H134R]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H134R mutation

   Promoter

   araBAD

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBT1-proD-mCherry-DAS+4

   Plasmid

   #65824

   Purpose

   Const. mCherry-DAS_4 expression , proD promoter, E. coli Expression

   Depositor

   Michael Lynch

   Insert

   mCherry-DAS+4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proD E . coli Constitutive Promoter

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCB301-2μ-HDV

   Plasmid

   #196329

   Purpose

   A yeast-Escherichia coli-Agrobacterium shuttle vector

   Depositor

   Zhenghe Li

   Article

   Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21b FAST-PETase

   Plasmid

   #225760

   Purpose

   Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   FAST-PETase
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGRB

   Plasmid

   #71539

   Purpose

   for expression of gRNA in E. coli

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J231196

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21b Hot-PETase

    Plasmid

    #225761

    Purpose

    Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    Hot-PETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRM006

    Plasmid

    #98922

    Purpose

    Express Histag-SUMO-FtsZ for purification in E coli

    Depositor

    Jie Xiao

    Article

    Yang et al Science. 2017 Feb 17;355(6326):744-747.

    Insert

    FtsZ
    

    Use

    Tags

    Histag-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBbS9a-RFP

    Plasmid

    #126172

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23113

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSU-araC-Cas9

    Plasmid

    #120423

    Purpose

    Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBbB19k-GFP

    Plasmid

    #126135

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-eGFP-MinD

    Plasmid

    #133622

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

    Depositor

    Petra Schwille

    Article

    Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

    Insert

    EGFP-MinD (minD E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET24c(+)-EFTu

    Plasmid

    #31172

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Tu (tufB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMUT2-pTcpP-mCherry

    Plasmid

    #192861

    Purpose

    pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJB153

    Plasmid

    #92328

    Purpose

    Express mEos3.2 tagged ZapA in E coli

    Depositor

    Jie Xiao

    Article

    Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

    Insert

    ZapA
    

    Use

    Tags

    mEos3.2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBbB19c-GFP

    Plasmid

    #126139

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbA13c-RFP

    Plasmid

    #126098

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge