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pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Use

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RHOA-G14V-deltaCAAX

Plasmid

#176097

Purpose

Constitutively active, nucelar localized RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RHOA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

G14V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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K365-iLid

Plasmid

#188456

Purpose

Component of optically-induced motor dimerization system

Depositor

Zvonimir Dogic

Article

Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

Insert

Kinesin 1-365 (Khc Fly)

Use

Tags

6xHis and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-365

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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K365-micro

Plasmid

#188457

Purpose

Component of optically-induced motor dimerization system

Depositor

Zvonimir Dogic

Article

Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

Insert

Kinesin 1-365 (Khc Fly)

Use

Tags

6xHis-MBP-TEV and SspB micro

Expression

Bacterial

Mutation

Kinesin amino acids 1-365

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1-2

Plasmid

#52378

Purpose

expresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Use

Tags

Expression

Mammalian

Mutation

GAGAL of syndecan-1 replaced with of GADED of SDC…

Promoter

CMV

Available since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
MSCV-AML1/ETO-IRES-GFP

Plasmid

#60832

Purpose

AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

AML1/ETO (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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shERK2b-mlpx-puro

Plasmid

#65230

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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shERK2a-mlpx-puro

Plasmid

#65229

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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shERK1a-mlpx-puro

Plasmid

#65228

Purpose

encodes a shRNA against ERK1

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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shERK2b-mlpx-neo

Plasmid

#65231

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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CUP Ent2 YFP p316

Plasmid

#15593

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

ENT2 (ENT2 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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CUP Rnq1 YFP p316

Plasmid

#15596

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

RNQ1 (RNQ1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
CUP Puf2 YFP p316

Plasmid

#15595

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

PUF2 (PUF2 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBAD-LSSmOrange

Plasmid

#37129

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

His

Expression

Bacterial

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

araB

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-3

Plasmid

#42131

Depositor

Vsevolod Belousov

Article

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-C1

Plasmid

#37131

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-N1

Plasmid

#37130

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
CUP Cyc8 YFP p316

Plasmid

#15594

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

CYC8 (CYC8 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
CUP Pan1 YFP p316

Plasmid

#15592

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

PAN1 (PAN1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
CUP Snf5 YFP p316

Plasmid

#15597

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

SNF5 (SNF5 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only