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pCS2 xenopus twisted gastrulation

Plasmid

#24974

Depositor

Edward De Robertis

Article

Larrain et al Development. 2001 Nov . 128(22):4439-47.

Insert

twisted gastrulation (Twsg1 Mouse)

Tags

FLAG and chordin signal peptide

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pGL2 enhancer- F3 Sp-1 mut

Plasmid

#22432

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F3 Sp-1 mut (NOS3 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only
EC.SurA.pTYB1.a21-428.L239R

Plasmid

#15957

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

surA (surA E. coli)

Tags

intein

Expression

Bacterial

Mutation

L239R

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf48

Plasmid

#12736

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf48

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pAG195 TNKS2 3'UTR

Plasmid

#11999

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

TNKS2 3'UTR (TNKS2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG187 RIPK4 3'UTR

Plasmid

#12063

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N20 3'UTR (RIPK4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-M.SssI (dCas9-M.SssI)

Plasmid

#218782

Purpose

Expresses dCAS9-M.SssI fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-M.SssI (dCas9-M.SssI)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-SunTag (dCas9-SunTag)

Plasmid

#218783

Purpose

Expresses dCAS9-SunTag fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-SunTag (dCas9-SunTag)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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hEDIL3-EGFP

Plasmid

#242341

Purpose

mammalian expression construct for human EGF-like repeats and discoidin I-like domains 3 C-terminally tagged with EGFP

Depositor

Christoph Kaether

Article

Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi

Insert

EGF-like repeats and discoidin I-like domains 3 (EDIL3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-IKZF3

Plasmid

#207217

Purpose

doxycycline-inducible expression of Human IKZF3 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-IKZF3 (IKZF3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pb-CAGGS-dCas9-VP64-blasto

Plasmid

#218556

Purpose

Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

dCas9

Use

CRISPR; Piggybac transposition

Tags

2xSV40NLS-VP64

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG1(WT)

Plasmid

#79431

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG1 (ARFGEF1 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Mutation

C1758R

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mGL(miRE.FF4)-bGH

Plasmid

#235264

Purpose

microRNA expression for dual-vector iFFL circuit

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK2(delta MTS)-3xFLAG

Plasmid

#233896

Purpose

lentiviral overexpression vector for NADK2 (delta MTS)

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK2 (NADK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

delta MTS (see Depositor Comments)

Promoter

Ubc

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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EK0007-PB-4XnrUAS-H2B-BFP

Plasmid

#236121

Purpose

Fuorescent reporter encoding H2B-tagBFP fusion under control of E1b minimal promoter with four Gal4-binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

E1b minimal promoter with four Gal4-binding sites…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW410 IKZF1tar x8-H2B-GFP

Plasmid

#236156

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with eight IKZF1 binding sit…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-eGFP-DDHD2

Plasmid

#232463

Purpose

Expresses GFP tagged DDHD2 lipase in neurons

Depositor

Timothy Ryan

Article

Kumar et al Nat Metab. 2025 Jul;7(7):1392-1403. doi:

Insert

DDHD2 (DDHD2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1 (SB251)

Plasmid

#227600

Purpose

Inducible expression of Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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CUP1-∆ssCPY*-mGFP in pRS315

Plasmid

#221158

Purpose

Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

Depositor

Mark Leake

Insert

Carboxypeptidase Y (PRC1 Budding Yeast)

Tags

mGFP

Expression

Yeast

Mutation

Deletion signal peptide, G255A mutation

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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