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We narrowed to 12,033 results for: LEA

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Showing: 11981 - 12000 of 12033 results

  1. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid
    #140455
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, leucine 624 to asparagine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140455-plasmid-map-sequence-id-292306

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  2. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid
    #140456
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, alanine 634 to asparagine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140456-plasmid-map-sequence-id-292300

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  3. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid
    #140452
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, isoluecine 578 to alanine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140452-plasmid-map-sequence-id-292301

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  4. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid
    #140453
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, phenylalanine 588 to val…
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140453-plasmid-map-sequence-id-292308

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  5. pHsSyx3-MBP

    Plasmid
    #179286
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    TEV protease cleavage site + mature maltose bindi…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179286-plasmid-map-sequence-id-354920

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  6. pMBP-HsSyx3

    Plasmid
    #179287
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179287-plasmid-map-sequence-id-354925

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  7. pαH-S-GSAS-B.1.351.v4

    Plasmid
    #173788
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 4
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    173788-plasmid-map-sequence-id-352110

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  8. pGBW-m4046774

    Plasmid
    #145574
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145574-plasmid-map-sequence-id-287751

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  9. pCEP4-BG505.SOSIP-gp160-QES.i05.c06-754*

    Plasmid
    #123275
    Purpose
    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (BG505) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; SOSIP mutations; …
    Promoter
    CMV
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    123275-plasmid-map-sequence-id-235239

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  10. pFUSEss-chimeric-IgM-mouse/human_M18

    Plasmid
    #91738
    Purpose
    Expression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Residues 203-239 (EU numbering) were exchanged to…
    Promoter
    hEF1-HTLV prom
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91738-plasmid-map-sequence-id-181015

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  11. pEG628

    Plasmid
    #40120
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)
    Use
    Gateway Cloning
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa1-244, Exon 2 recoded to be RNAi-resis…
    Promoter
    4.4 kb mex-5 promoter fragment
    Available Since
    Sept. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  12. pET30-GBF-E6

    Plasmid
    #21696
    Depositor
    James Baleja
    Article
    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.
    Insert
    protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)
    Tags
    Protein G B1 domain
    Expression
    Bacterial
    Mutation
    GB1 (1-56aa) fusion tag with mutations at three p…
    Available Since
    July 28, 2009
    Availability
    Academic Institutions and Nonprofits only

  14. pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-P2A-EGFP-LMNB1

    Plasmid
    #250905
    Purpose
    Dual color expression of mKate2 tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-P2A-EGFP-Lamin B1 (lmnb1, ARFGAP1 Human, Zebrafish)
    Tags
    EGFP on LMNB1 and mKate2 on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250905-plasmid-map-sequence-id-498468

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  15. pSB-CMV-MCS-Puro-ArfGAP1_ALPS1-mKate2-3NLS-P2A-eGFP-LMNB1

    Plasmid
    #250914
    Purpose
    Dual color expression of mKate2-3xNLS tagged rat ALPS1 and eGFP tagged zebrafish Lamin B1 in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-LMNB1 (lmnb1, Arfgap1 Rat, Zebrafish)
    Tags
    EGFP on LMNB1 and mKate2 on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 196–234 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250914-plasmid-map-sequence-id-498467

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  16. pCMV-T7-TadCBEd-SpCas9(MRKQKC)-P2A-EGFP (AHK574)

    Plasmid
    #223134
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MRKQKC)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MRKQKC)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223134-plasmid-map-sequence-id-447700

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  17. pCMV-T7-TadCBEd-SpCas9(MRKSKC)-P2A-EGFP (AHK446)

    Plasmid
    #223135
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MRKSKC)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MRKSKC)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223135-plasmid-map-sequence-id-447701

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  18. pCMV-T7-TadCBEd-SpCas9(KWRQLC)-P2A-EGFP (BKS1026)

    Plasmid
    #223126
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(KWRQLC)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/KWRQLC)=D1135K, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223126-plasmid-map-sequence-id-447694

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  19. pCMV-T7-TadCBEd-SpCas9(LWRYEK)-P2A-EGFP (BKS1022)

    Plasmid
    #223128
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWRYEK)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWRYEK)=D1135L, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223128-plasmid-map-sequence-id-447696

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  20. pCMV-T7-TadCBEd-SpCas9(MKRCMV)-P2A-EGFP (BKS1032)

    Plasmid
    #223129
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MKRCMV)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MKRCMV)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223129-plasmid-map-sequence-id-447698

    Enlarge

Showing: 11981 - 12000 of 12033 results