We narrowed to 15,769 results for: LEA

1. pAAV-synapsin-HaloCaMP1a-EGFP

   Plasmid

   #138327

   Purpose

   chemigenetic calcium indicator

   Depositor

   Eric Schreiter

   Article

   Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

   Has Service

   AAV1 and AAV9

   Insert

   HaloCaMP1a
   

   Use

   AAV

   Tags

   EGFP, His-tag, and Nuclear Exclusion Signal

   Expression

   Mammalian

   Promoter

   synapsin

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-NLS-miRFP670nano-miRFP670

   Plasmid

   #216131

   Purpose

   Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ExRai-CKAR(T/A)

   Plasmid

   #118410

   Purpose

   Negative-control mutant for ExRai-CKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-CKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSFFV-dNme2Cas9

   Plasmid

   #193190

   Purpose

   Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   dNme2Cas9
   

   Tags

   NLS and NLS-3xHA-NLS

   Expression

   Mammalian

   Mutation

   H588A, D16A

   Promoter

   SFFV

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pre_IV_CS10_1545_v1

   Plasmid

   #126861

   Purpose

   4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS10_1545_v1
   

   Use

   Synthetic Biology

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pre_IV_CS10_1033_v1

   Plasmid

   #126860

   Purpose

   4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS10_1033_v1
   

   Use

   Synthetic Biology

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEY11

   Plasmid

   #191017

   Purpose

   Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   Ultrapanneuronal
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

   Plasmid

   #111581

   Purpose

   An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   myrTagRFP-T-P2A-post-eGRASP
   

   Use

   AAV

   Promoter

   Ef1a

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE60 R130A wgMDH

    Plasmid

    #204266

    Purpose

    Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-191084-gp160

    Plasmid

    #123234

    Purpose

    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (191084 B7-19) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE60 R124A wgMDH

    Plasmid

    #204257

    Purpose

    Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNCS mStrawberry

    Plasmid

    #91768

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Strawberry
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE60 R196A wgMDH

    Plasmid

    #204271

    Purpose

    Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-calpainsensor

    Plasmid

    #36182

    Purpose

    detection of activity of ubiquitous calpains

    Depositor

    Isabelle Richard

    Article

    Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

    Insert

    alpha-Fodrin cleavage site (Sptan1 Mouse)
    

    Tags

    eCFP and eYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a hMDH2

    Plasmid

    #204230

    Purpose

    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH2 (MDH2 Human)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Does not include transit peptide and includes a M…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG-16-3xFLAG-LaG-2

    Plasmid

    #172765

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-3xFLAG-LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AA012

    Plasmid

    #215974

    Purpose

    Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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