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Showing: 11981 - 12000 of 19471 results

  1. pLCHKOv3-intergenic-As

    Plasmid
    #209026
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209026-plasmid-map-sequence-id-413692

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  2. pLCHKOv3-intergenic-Lb

    Plasmid
    #209030
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209030-plasmid-map-sequence-id-413690

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  4. pL0_AC-27877

    Plasmid
    #202110
    Purpose
    Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Publication
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    27877 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Available since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202110-plasmid-map-sequence-id-405522

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  5. pL0_AC-Fld

    Plasmid
    #202125
    Purpose
    Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Publication
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Fld promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Available since
    Aug. 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202125-plasmid-map-sequence-id-405541

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  6. pL0_AC-54730

    Plasmid
    #202109
    Purpose
    Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Publication
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    54730 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202109-plasmid-map-sequence-id-405433

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  7. pL0_AC-51183

    Plasmid
    #202108
    Purpose
    Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Publication
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    51183 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202108-plasmid-map-sequence-id-405432

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  8. pL0_AC-GEF

    Plasmid
    #202107
    Purpose
    Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Publication
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    GEF promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI site for…
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202107-plasmid-map-sequence-id-405430

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  9. pL0_AC-47740

    Plasmid
    #202106
    Purpose
    Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Publication
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    47740 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202106-plasmid-map-sequence-id-405429

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  10. pL0_AC-BCA

    Plasmid
    #202105
    Purpose
    Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Publication
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    BCA promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202105-plasmid-map-sequence-id-405428

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  11. pL0_AC-epyC

    Plasmid
    #202104
    Purpose
    Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Publication
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    epyC promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI sites fo…
    Available since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202104-plasmid-map-sequence-id-405424

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  13. ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA

    Plasmid
    #178108
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178108-plasmid-map-sequence-id-350191

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  15. pLCHKO_HPRT1_exon3_2_As

    Plasmid
    #155058
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155058-plasmid-map-sequence-id-308441

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  16. pLCHKO_HPRT1_exon2_2_As

    Plasmid
    #155056
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155056-plasmid-map-sequence-id-308440

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  17. pLCHKO_HPRT1_exon2_1_As

    Plasmid
    #155055
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155055-plasmid-map-sequence-id-308438

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  18. pLCHKO_HPRT1_exon2_2_Lb

    Plasmid
    #155052
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155052-plasmid-map-sequence-id-308434

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Showing: 11981 - 12000 of 19471 results