We narrowed to 4,343 results for: Ado;

1. EF1a_SNAI2_P2A_Hygro_Barcode

   Plasmid

   #120478

   Purpose

   Barcoded lentiviral vector to express SNAI2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SNAI2 (SNAI2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO mScarlet

   Plasmid

   #131002

   Purpose

   Double floxed mScarlet under the control of Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV1 and AAV5

   Insert

   mScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a_MITF_P2A_Hygro_Barcode

   Plasmid

   #120460

   Purpose

   Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MITF (MITF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-fDIO-mNeonGreen

   Plasmid

   #99133

   Purpose

   An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   fDIO-mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPTF PHD and Bromodomain

   Plasmid

   #92101

   Purpose

   Expresses GST fusion PHD and Bromodomain from BPTF protein

   Depositor

   Albert Jeltsch

   Article

   Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

   Insert

   PHD and Bromodomain (BPTF Human)
   

   Use

   Tags

   GST Tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF-GalNAc-T2-msGFP2

   Plasmid

   #140164

   Purpose

   Expresses the Golgi marker GalnacT2-msGFP2

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   GalNAc-T2-msGFP2 (GALNT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. VN-beta Synuclein

   Plasmid

   #89483

   Purpose

   expresses human beta Synuclein fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

   Insert

   SNCB (SNCB Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2xHP1bCD_W42A-IFP2.0C

   Plasmid

   #215751

   Purpose

   Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   2xHP1bCD_W42A-IFP2.0C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW212

   Plasmid

   #172196

   Purpose

   P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

   Depositor

   Chang Liu

   Article

   Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

   Insert

   p10B2-AT110
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   10B2 (P1 specific promoter)

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HotPETase_pET21b

    Plasmid

    #215407

    Purpose

    Expression construct for HotPETase gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    HotPETase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_NEUROD1_P2A_Hygro_Barcode

    Plasmid

    #120466

    Purpose

    Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    NEUROD1 (NEUROD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC152-dual-dCas9VP64-sgExpression

    Plasmid

    #48238

    Purpose

    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA Tag, HA-Tag, and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL1-SpCas9

    Plasmid

    #117051

    Purpose

    In vitro expression of S. pyogenes Cas9 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    S. pyogenes Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. FAM177A1isoform2-GFP

    Plasmid

    #224589

    Purpose

    For expression of FAM177A1-GFP in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    FAM177A1 (FAM177A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Arl6ip1-mEmerald

    Plasmid

    #186965

    Purpose

    Express Arl6ip1 with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    ARL6IP1 (ARL6IP1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. VPS13B^GFP

    Plasmid

    #224588

    Purpose

    codon optimized GFP tagged VPS13B for expression in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    VPS13B^GFP (VPS13B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRN3P-NLS-EGFP-deadBtgh-3NLS

    Plasmid

    #194470

    Purpose

    Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

    Depositor

    Mathieu Boulard

    Article

    Formichetti et al Dev Cell 10 Jan 2024

    Insert

    BtGH84
    

    Use

    For in vitro transcription from t3 promoter after…

    Tags

    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

    Expression

    Mutation

    Aspartic Acid 242 (in Dennis et al.) changed to A…

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-SypHer2-DAAO-NES

    Plasmid

    #119184

    Purpose

    Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

    Depositor

    Thomas Michel

    Article

    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

    Insert

    SypHer2-DAAO-NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-GFAP-mKate2.5f

    Plasmid

    #99129

    Purpose

    Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mKate2.5f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+ Zf GFP-vclb

    Plasmid

    #185411

    Purpose

    GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin b (vclb Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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