We narrowed to 3,045 results for: ER;

1. pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

   Plasmid

   #179717

   Purpose

   Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET SUMO-FL-syt7

   Plasmid

   #213630

   Purpose

   Bacterial expression of SUMO-tagged mouse FL-syt7

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt7 (Syt7 Mouse)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA

   Plasmid

   #201964

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   E162A,M164A,R167A

   Available Since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

   Plasmid

   #201965

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   E198A, R199A,E200A, D201A

   Available Since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

   Plasmid

   #201967

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   R232A, K233A, R234A, K235A, R236A

   Available Since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

   Plasmid

   #201968

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   W255A,N262E,K266E

   Available Since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI pHluorin Syt6

   Plasmid

   #184507

   Purpose

   Mammalian expression of Synaptotagmin 6 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 6 (Syt6 Mouse)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETmsGFPSyt1(80 JuxtaK-142)

   Plasmid

   #209013

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis-msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETSUMOSyt1C2AB(80 JuxtaK-421)

   Plasmid

   #209008

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209016

    Purpose

    Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209011

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Tags

    6xHis and msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX SYT9 C2A D197,199N

    Plasmid

    #195704

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 124-211 of mouse SYT9 with D1…

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX SYT9 C2B D330, 332N

    Plasmid

    #195705

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 253-344 of mouse SYT9 with D3…

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSC-cUnaG

    Plasmid

    #207631

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-cUnaG
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSC-LPETG

    Plasmid

    #207629

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-LPETG
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SiRES COR1C ACT-DCC-Halo

    Plasmid

    #195148

    Purpose

    image COR1C

    Depositor

    Gia Voeltz

    Article

    Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    CORO1C (CORO1C Human)
    

    Expression

    Mammalian

    Mutation

    mutations to make si RNA resistant and remove act…

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SiRES COR1C ACT-DCC-GFP

    Plasmid

    #195147

    Purpose

    image COR1C

    Depositor

    Gia Voeltz

    Article

    Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    CORO1C (CORO1C Human)
    

    Expression

    Mammalian

    Mutation

    mutations to make si RNA resistant and remove act…

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-RedO-Txnip(C247S)

    Plasmid

    #206341

    Purpose

    AAV plasmid expressing mutant TXNIP in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Mutation

    C247S

    Promoter

    human red opsin

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFsynW SYT1-PGM reporter

    Plasmid

    #197278

    Purpose

    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI pHluorin Syt17

    Plasmid

    #184514

    Purpose

    Mammalian expression of Synaptotagmin 17 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 17 (Syt17 Rat)
    

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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