Addgene

We narrowed to 1,302 results for: ckm

Showing: 1201 - 1220 of 1302 results

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pEMS1538

Plasmid

#29261

Purpose

High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple67 (FEV Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

Oct. 28, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiCRISPR V2 Neo sgITGB1_3

Plasmid

#233250

Purpose

Knock out of murine ITGB1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

ITGB1 (Itgb1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 Neo sgITGB1_2

Plasmid

#233249

Purpose

Knock out of murine ITGB1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

ITGB1 (Itgb1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

Plasmid

#213527

Purpose

Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

D90A and E92A

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_hRNMT_RAM

Plasmid

#213528

Purpose

Expresses the human RNMT-RAM complex

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Inserts

human RNMT (RNMT Human)
RAM

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14

Plasmid

#213518

Purpose

Expresses NSP14 of SARS-CoV-2

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

N-terminal His6-sumo

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_OC43_NSP14

Plasmid

#213519

Purpose

Expresses NSP14 of hCoV OC43

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV OC43 NSP14 (ORF1ab hCoV OC43)

Use

Tags

His-SUMO tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_NL63_NSP14

Plasmid

#213520

Purpose

Expresses NSP14 of hCoV NL63

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV NL63 NSP14 (ORF1ab hCoV NL63)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_229E_NSP14

Plasmid

#213521

Purpose

Expresses NSP14 of hCoV 229E

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV 229E NSP14

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

Plasmid

#213522

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed V290 to A

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

Plasmid

#213523

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed N306 to K

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

Plasmid

#213524

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to S

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Use

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-PSD [N286/74R]

Plasmid

#164159

Purpose

Mammalian Expression Plasmid of anti-PSD (Zebrafish). Derived from hybridoma N286/74.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-PSD (Danio rerio) recombinant mouse monoclonal antibody (dlg2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.3 K+ channel [N375/67R]

Plasmid

#182113

Purpose

Mammalian Expression Plasmid of anti-Kv3.3 K+ channel (Mouse). Derived from hybridoma N375/67.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv3.3 K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnc3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-DNAH7 [N250/21R]

Plasmid

#206547

Purpose

Mammalian Expression Plasmid of anti-DNAH7 (Human). Derived from hybridoma N250/21.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-DNAH7 (Homo sapiens) recombinant Mouse monoclonal antibody (DNAH7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta PBM

Plasmid

#202415

Purpose

Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Lacking DTHL

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL FBM

Plasmid

#202417

Purpose

Expression of GFP-tagged PODXL FERM-binding motif mtuation (FBM; doesn't bind Ezrin)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

H477/R479/S481>AAA mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta PBM + Ezrin

Plasmid

#202418

Purpose

Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

Ezrin and GFP

Expression

Mutation

delete PDZ binding motif

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Showing: 1201 - 1220 of 1302 results

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