161,152 results

1. pLR5-CBh-dCas9-hEzh2-IRES-Hyg

   Plasmid

   #122375

   Purpose

   Expresses dCas9-hEzh2-IRES-Hyg in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   CBh-dCas9-hEzh2-IRES-Hyg-pA
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AY22_pgRNA.R5.1

   Plasmid

   #100294

   Purpose

   CCR5-targeting gRNA expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 RNA Pol III promoter human snRNA

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV8R-12 Capsid

   Plasmid

   #213968

   Purpose

   AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBES1-LacOx50-Hyg

   Plasmid

   #210261

   Purpose

   Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   50xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDS246

   Plasmid

   #43861

   Purpose

   Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

   Depositor

   Keith Joung

   Insert

   mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
   

   Use

   CRISPR

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4XCLEAR-luciferase reporter

   Plasmid

   #66800

   Purpose

   Reporter for activity of the CLEAR network

   Depositor

   Albert La Spada

   Article

   Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

   Insert

   4XCLEAR
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Four CLEAR elements (GTCACGTGAC) in tandem derive…

   Available since

   Nov. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO-oG-WPRE-hGH

   Plasmid

   #74290

   Purpose

   pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   Ef1a

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-anti-GFP(LaG2) PAGER(TF)

   Plasmid

   #229996

   Purpose

   Expresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   IL2SP-Aro6-LaG2-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   V360A/R361A on KORD

   Promoter

   CMV

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL

   Plasmid

   #196286

   Purpose

   35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV L antigenome encoding codon-optimized L (RdRp)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2C::tdTomato Reporter

    Plasmid

    #40281

    Depositor

    Samuel Pfaff

    Article

    Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

    Insert

    2C Promoter (2-cell stage promoter)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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