178,346 results
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Plasmid#108246PurposeLentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFPDepositorInsertdCas9-dead Tet1CD-HA-P2A-BFP
UseLentiviralTagsHAExpressionMammalianMutationdCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…PromoterUbcAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ELONGIN BC (XX01TCEB1A-c001)
Plasmid#110274PurposeElongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin CDepositorExpressionBacterialMutationElongin B is full length. Elongin C contains a.a…PromoterT7Available sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-AviTag-SnoopLigase
Plasmid#105626PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylationDepositorInsertAviTag-SnoopLigase
ExpressionBacterialPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPBbsr2-4031NES
Plasmid#105241PurposeA Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK-EV
ExpressionMammalianPromoterCAGGSAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)
Plasmid#103849PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterCMVAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti-EF1a-dCas9-VP64-Puro
Plasmid#99371Purpose3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)DepositorInsertdCas9-VP64-T2A-Puro
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
htrpC6-YFP
Plasmid#21084DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrpC6 (TRPC6 Human)
TagsYFPExpressionMammalianAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
UseCRISPRAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP
Plasmid#86827PurposeExpresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAsDepositorInsertFLAG-NLS-MS2-eGFP
ExpressionMammalianPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
ExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by